PARCLIP analysis of NONO and SFPQ from U2OS and Hela cells

ABSTRACT: To identify the RNA targets of the RNA binding proteins NONO and SFPQ in two different cell types, PARCLIP was carried out (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation) using a monoclonal antibody that recognises a NONO/SFPQ heterodimer. In this analysis, only the target gene NEAT1 was analysed, to show the binding sites for NONO and SFPQ in this transcript in the two cell lines.

ORGANISM(S): Homo sapiens

PROVIDER: GSE113349 | GEO | 2018/06/27

REPOSITORIES: GEO