Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome of Gossypium hirsutum floral and extrafloral nectaries

ABSTRACT: Comparative RNA-Seq study of expression in Gossypium hirsutum nectaries

ORGANISM(S): Gossypium hirsutum

PROVIDER: GSE113373 | GEO | 2018/04/19

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome of Gossypium hirsutum floral and extrafloral nectaries

Project description:Transcriptome of Gossypium hirsutum floral and extrafloral nectaries

| PRJNA450940 | ENA

Transcriptomics and Metabolomics of 10 - 28 day post anthesis cotton fiber from Gossypium hirsutum and Gossypium barbadense

2015-06-26 | GSE64958 | GEO

Transcriptomics and Metabolomics of 10 - 28 day post anthesis cotton fiber from Gossypium hirsutum and Gossypium barbadense

Project description:Purpose: The goal of this experiment was to use RNA-seq to compare the two commercial cotton species Gossypium hirsutum and Gossypium barbadense and determine what transcripts may account for the better fiber quality in the latter. Methods: RNA was extracted from Gossypium barbadense or Gossypium hirsutum fibers at 10, 15, 18, 21, and 28 days post anthesis. Paired-end, 100-bp RNA-seq was performed on an Illumina HiSeq2000 and the reads were mapped to the Gossypium raimondii genome at www.phytozome.net and non-homologous contig assemblies from Gossypium arboreum. Results from RNA-seq were combined with non-targeted metabolomics. Results: Approximately 38,000 transcripts were expressed (RPKM>2) in each fiber type and approximately 2,000 of these transcripts were differentially expressed in a cross-species comparison at each timepoint. Enriched Gene Ontology biological processes in differentially expressed transcripts suggested that Gh fibers were more stressed. Conclusions: Both metabolomic and transcriptomic data suggest that better mechanisms for managing reactive oxygen species contribute to the increased fiber length in Gossypium barbadense. This appears to result from enhanced ascorbate biosynthesis via gulono-1,4-lactone oxidase and ascorbate recycling via dehydroascorbate reductase. See Bioproject PRJNA263926 and SRA accession SRP049330 for study design and raw sequencing data and Bioproject PRJNA269608 and TSA accession GBYK00000000 for Gossypium arboreum assembled contig sequences used for transcriptome mapping - Cotton fiber mRNA from 10,15,18,21 and 28 day post anthesis fiber from either Gossypium hirusutm or Gossypium barbadense was sequenced and differential gene expression analysis was conducted between species for each timepoint and between adjacent timepoints. Each timepoint was representative of fiber from 9 individual plants processed as 3 biological replicate pools (material from 3 individual plants per pool).

2015-06-26 | E-GEOD-64958 | biostudies-arrayexpress

Transcriptome of Phaseolus vulgaris nectaries and leaves

Project description:Comparative RNA-Seq study of expression in Phaseolus vulgaris nectaries

2018-04-10 | GSE112944 | GEO

Transcriptome of Brassica juncea nectaries and leaves

Project description:Comparative RNA-Seq study of expression in B.juncea nectaries from different lines.

2018-04-04 | GSE112675 | GEO

Gossypium hirsutum

Project description:Gossypium hirsutum

| PRJNA168346 | ENA

Global gene expression analysis of cotton (Gossypium hirsutum L.) under drought stress in leaf tissue and during fibre development stages.

Project description:This SuperSeries is composed of the following subset Series: GSE29566: Global gene expression analysis of cotton (Gossypium hirsutum L.) under drought stress in leaf tissue. GSE29567: Global gene expression analysis of cotton (Gossypium hirsutum L.) under drought stress during fibre development stages. Refer to individual Series

2012-06-03 | E-GEOD-29810 | biostudies-arrayexpress

Transcriptome of sunflower (Helianthus annuus) nectaries and leaf

Project description:Comparative RNA-Seq study of expression in sunflower nectaries from different lines.

2018-04-03 | GSE112583 | GEO

Gossypium hirsutum

Project description:Gossypium hirsutum transcriptome

| PRJNA301536 | ENA

Gossypium hirsutum

Project description:Gossypium hirsutum degradome

| PRJNA264880 | ENA