Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

IL17 and IL22 stimulation of T84 colonic epithelial cell line

ABSTRACT: The effects of stimulating intestinal epithelial cells with Th17 cytokines, IL17 and IL22, was investigated Keywords: dose response

ORGANISM(S): Homo sapiens

PROVIDER: GSE11345 | GEO | 2008/05/07

SECONDARY ACCESSION(S): PRJNA106559

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of human T84 colonic epithelial cell line response to IL17 and IL22 stimulation

Project description:The effects of stimulating intestinal epithelial cells with Th17 cytokines, IL17 and IL22, was investigated Experiment Overall Design: The human colonic epithelial cell line, T84 was grown to confluency in standard transwell plates and either mock treated, or treated with cytokines IL17 and IL22

2008-06-20 | E-GEOD-11345 | biostudies-arrayexpress

Transcriptome profile of NON CF (normal) bronchial epithelial cells treated with TNFalpha, IL17 and TNFalpha+IL17

Project description:To analyse gene expression differences between differentiated bronchial epithelial cells kept under control conditions or treated for 72 hours with cytokines (TNFalpha10 ng/ml; IL17 20ng/ml)

2022-10-19 | GSE182957 | GEO

Next Generation Sequencing Facilitates analysis of IL17 EGFP positive and negative cells from small intestine (SI) lamina propia (lp) genes expression

Project description:We performed the bulk RNA seq on IL17 EGFP positive and negative cells from small intestine (SI) lamina propia (lp) of antibiotic treated XBP1ΔIEC mice and found the different genes expression in the setting of IEC ER stress induced Th17 positive and negative cells

2023-03-13 | GSE203537 | GEO

Analysis of the function of IL17 in C.elegans

Project description:RNAseq of C.elegans mutants of varying IL17 loss and gain of function mutants

2022-07-08 | E-MTAB-11701 | biostudies-arrayexpress

BET bromodomain inhibition suppresses TH17-mediated pathology

Project description:IL-17-producing T helper (TH17) cells have been selected through evolution for their ability to control fungal and bacterial infections. It is also firmly established that their aberrant generation and activation results in autoimmune conditions. Using a characterized potent and selective small molecule inhibitor, we show that the bromodomain and extra-terminal domain (BET) family of chromatin adaptors plays fundamental and selective roles in human and murine TH17 differentiation from naM-CM-/ve CD4+ T cells, as well as in the activation of previously differentiated TH17 cells. We provide evidence that BET controls TH17 differentiation in a bromodomain-dependent manner through a mechanism that includes the direct regulation of multiple effector TH17-associated cytokines, including IL17, IL21 and GMCSF. We also demonstrate that BET family members Brd2 and Brd4 associate with the Il17 locus in TH17 cells, and that this association requires bromodomains. We recapitulate the critical role of BET bromodomains in TH17 differentiation in vivo and show that therapeutic dosing of the BET inhibitor is efficacious in mouse models of autoimmunity. Our results identify the BET family of proteins as a fundamental link between chromatin signaling and TH17 biology, and support the notion of BET inhibition as a point of therapeutic intervention in autoimmune conditions. 4 samples were analyzed: two conditions in duplicate. Naive T cells were placed in conditions leading to TH17 differentiation, with and without BET inhibitor. RNA was collected at 48 hours.

2013-09-20 | E-GEOD-37270 | biostudies-arrayexpress

anti-IL17 in RA

Project description:RNAseq of biopsies of RA patients treated with anti-IL17

| PRJEB40928 | ENA

BET bromodomain inhibition suppresses TH17-mediated pathology

Project description:IL-17-producing T helper (TH17) cells have been selected through evolution for their ability to control fungal and bacterial infections. It is also firmly established that their aberrant generation and activation results in autoimmune conditions. Using a characterized potent and selective small molecule inhibitor, we show that the bromodomain and extra-terminal domain (BET) family of chromatin adaptors plays fundamental and selective roles in human and murine TH17 differentiation from naïve CD4+ T cells, as well as in the activation of previously differentiated TH17 cells. We provide evidence that BET controls TH17 differentiation in a bromodomain-dependent manner through a mechanism that includes the direct regulation of multiple effector TH17-associated cytokines, including IL17, IL21 and GMCSF. We also demonstrate that BET family members Brd2 and Brd4 associate with the Il17 locus in TH17 cells, and that this association requires bromodomains. We recapitulate the critical role of BET bromodomains in TH17 differentiation in vivo and show that therapeutic dosing of the BET inhibitor is efficacious in mouse models of autoimmunity. Our results identify the BET family of proteins as a fundamental link between chromatin signaling and TH17 biology, and support the notion of BET inhibition as a point of therapeutic intervention in autoimmune conditions.

2013-09-20 | GSE37270 | GEO

Homo sapiens

Project description:IL17 and IL22 stimulation of T84 colonic epithelial cell line

| PRJNA106559 | ENA

Characterization of the IL23-IL17 immune axis in IBD patients

Project description:The aim of this study is to investigate the molecular mechanisms of IL23-IL17 immune axis in IBD, with particular attention to its role in maintaining mucosal barrier integrity under both physiological and pathological conditions

2021-11-26 | GSE165512 | GEO

The T84 human colonic adenocarcinoma cell line produces mucin in culture and releases it in response to various secretagogues.

Project description: Not available

| S-EPMC1131316 | biostudies-other