Dataset Information


Cell culture-based model system for FOXO3 activity

ABSTRACT: MCF7 and MDA-MB-231 breast cancer cell lines were cultured in DMEM-F12 containing 10% FBS (Lonza) 100U/ml penicillin and 100 µg/ml streptomycin (Lonza). Transfecting third generation packaging vectors using Poly-ethylenimine into HEK293T cells generated lentiviral particles (17). MCF7 and MDA-MB-231 cells were stably transduced with lentivirus containing pINDUCER20-FOXO3.A3, allowing doxycycline induced expression of constitutively active FOXO3 (FOXO3.A3). Cells were treated with 20% FBS or 10 µM PI3K inhibitor LY294002 (Selleckchem) for 16 hours to activate and inactivate the endogenous PI3K pathway, respectively. FOXO3.A3 expression was induced by 16 hours treatment with 10 ng/ml doxycycline. Overall design: 3 biological replicates per condition; each experiment contained at least a control and a stimulated condition

INSTRUMENT(S): [HT_HG-U133_Plus_PM] Affymetrix HT HG-U133+ PM Array Plate (custom CDF)

SUBMITTER: Henk van Ooijen  

PROVIDER: GSE113479 | GEO | 2018-06-06


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Assessment of Functional Phosphatidylinositol 3-Kinase Pathway Activity in Cancer Tissue Using Forkhead Box-O Target Gene Expression in a Knowledge-Based Computational Model.

van Ooijen Henk H   Hornsveld Marten M   Dam-de Veen Christa C   Velter Rick R   Dou Meng M   Verhaegh Wim W   Burgering Boudewijn B   van de Stolpe Anja A  

The American journal of pathology 20180706 9

The phosphatidylinositol 3-kinase (PI3K) pathway is commonly activated in cancer. Tumors are potentially sensitive to PI3K pathway inhibitors, but reliable diagnostic tests that assess functional PI3K activity are lacking. Because PI3K pathway activity negatively regulates forkhead box-O (FOXO) transcription factor activity, FOXO target gene expression is inversely correlated with PI3K activity. A knowledge-based Bayesian computational model was developed to infer PI3K activity in cancer tissue  ...[more]

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