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DHX15 regulates CMTR1-dependent gene expression and cell proliferation

ABSTRACT: We discovered that the RNA helicase DHX15 is a regulator of the methyltransferase CMTR1. To determine the biological consequences of this interaction we overexpressed CMTR1 or a mutated CMTR1 (2LA)that does not bind DHX15, in the human breast cancer cell line HCC1806. To assess the effects of the DHX15-CMTR1 interaction on translation, polysomes were separated on a sucrose gradient and sequencing libraries generated from the polysomal and input RNA. We found that the DHX15-CMTR1 interaction controls ribosome loading of a subset of mRNAs and impacts on cell proliferation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE113573 | GEO | 2018/06/05

REPOSITORIES: GEO

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DHX15 regulates CMTR1-dependent gene expression and cell proliferation

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