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MS2-affinity purification coupled with RNA sequencing (MAPS) revealed MicF sRNA targetome.

ABSTRACT: In this study, we performed MAPS (MS2-affinity purification coupled with RNA sequencing) to draw the interacting map of MicF small regulatory RNA in vivo.

ORGANISM(S): Escherichia coli K-12

PROVIDER: GSE113584 | GEO | 2023/03/05

REPOSITORIES: GEO

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MS2-affinity purification coupled with RNA sequencing (MAPS) reveals RprA sRNA targetome.

Project description:Recently, we developed an in vivo technology to draw the interacting map of a specific small regulatory RNA (sRNA). We called it MAPS for MS2-affinity purification coupled with RNA sequencing. Using this technology, we already revealed the targetome of RyhB, RybB and DsrA, three well-characterized sRNAs in Escherichia coli. In this study, we perform MAPS with RprA sRNA.

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MS2-affinity purification coupled with RNA sequencing (MAPS) reveals S. aureus RsaA sRNA targetome

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