Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell transcriptomics identifies cellular heterogeneity in large skin wounds

ABSTRACT: We report transcriptomes of cells from large skin wounds. Dead cells were removed from post-wounding day (PWD) 12 skin wound tissue using dead cell removal kit (MACS). Remaining live cells were analyzed

ORGANISM(S): Mus musculus

PROVIDER: GSE113854 | GEO | 2018/12/05

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Single-cell transcriptomics confirms heterogeneity of contractile cells in large skin wounds

Project description:We report transcriptomes of pre-sorted skin wound dermal cells. Post-wounding day (PWD) 12, 15 and 21 Zombie-neg;tdTomatoHi cells were FACS sorted from Sm22-Cre;TdTomato mice.

2018-12-05 | GSE113605 | GEO

Heparan sulfate in skin wounds

Project description:Standardized skin wounds were established surgically on mice and allowed to heal during a 15-day period. Expression of genes related to heparan sulfate biosynthesis was studied in wound bed and edges during the healing process. Total RNA was isolated from wound edge (regenerating skin) and wound bed at 2, 6 and 15 days post wounding, as well as from intact control skin. Three animals were used for each time point.

2010-05-22 | E-GEOD-7435 | biostudies-arrayexpress

Single cell RNA-seq analyses of dermis from wound day 18 large skin wounds that healed in a fibrotic or regenerative fashion.

Project description:Human and murine skin wounding commonly result in fibrotic scarring but the murine wounding model Wound Induced Hair Neogenesis (WIHN) can frequently result in a regenerative repair response. Here we show in single cell RNA-seq comparisons of semi-regenerative and fibrotic WIHN wounds, increased expression of phagocytic/lysosomal genes in macrophages associated with predominance of fibrotic myofibroblasts in fibrotic wounds. Investigation revealed that macrophages in the late wound drive fibrosis by phagocytizing dermal Wnt inhibitor SFRP4 to establish persistent Wnt activity. In accordance, phagocytosis abrogation resulted in transient Wnt activity and a more regenerative healing. Phagocytosis of SFRP4 was integrin-mediated and dependent on the interaction of SFRP4 with the EDA splice variant of fibronectin. In the human skin condition Hidradenitis suppurativa, phagocytosis of SFRP4 by macrophages correlated with fibrotic wound repair. These results reveal that macrophages can modulate a key signaling pathway via phagocytosis to alter the skin wound healing fate.

2019-12-12 | GSE141814 | GEO

Expression profiling of WT and aSMA-TK wounds

Project description:RNA was isolated from WT and aSMA-TK wounds at day 6 and day 17 post-wounding

2022-01-10 | GSE178902 | GEO

Single-cell transcriptomics identifies cellular heterogeneity in large skin wounds

Project description:Single-cell transcriptomics identifies cellular heterogeneity in large skin wounds

| PRJNA454278 | ENA

RNA-seq of mouse wounds from different time points, treatment and genotype

Project description:We investigated transcriptional changes in wounds of wild-type and transgenic mice with either a lack or an overabundance of skin lymphatic vessels. Further, we investigated transcriptional changes in diabetic wounds of db/db mice treated with either F8-SIP or F8-VEGF-C.

2023-02-07 | E-MTAB-12545 | biostudies-arrayexpress

Single-cell ATAC-Seq of cells recruited to regenerative portions of large skin wounds.

Project description:Adult mammalian skin wound healing is typically accompanied by a fibrotic scar that impairs normal skin function and regeneration of skin appendages. Interestingly, however, in adult mice, large skin injuries exhibit de novo formation of hair follicles (HFs, a phenomenon termed wound-induced HF neogenesis) in the center of the wound. Our previous analysis provides compelling evidence suggesting that regional epigenetic changes within the mesenchymal cells of the skin may underlie the divergent response to wound healing. To test this directly, we performed single-cell Assay for Transposase-Accessible Chromatin using Sequencing (sc-ATAC-Seq) on cells isolated from the center of large wounds to identify regions of the genome that are becoming differentially accessible within upper (epithelial-interfacing) fibroblasts as they transition to induce new HFs compared to their lower dermal counterparts that adopt a fibrotic phenotype. Together, our data reveals the identity and dynamics of key coding, non-coding, and regulatory regions that underlie a wound responsive fibroblasts' transition to inductive neodermal condensate cells.

2020-08-04 | GSE131600 | GEO

Transcriptional profiling of macrophages in skin wounds

Project description:Macrophages were isolated from wounded skin 4 and 14 days post wounding. Monocytes were adoptively transferred on days 2 and 12 and traced into the wound.

2021-10-28 | GSE183489 | GEO

Heparan sulfate in skin wounds

2008-04-01 | GSE7435 | GEO

Identification of a myofibroblast-specific expression signature in skin wounds

Project description:After skin injury fibroblasts migrate into the wound and transform into contractile extracellular matrix-producing myofibroblasts to promote skin repair. Persistent activation of myofibroblasts can cause excessive fibrotic reactions, but the underlying mechanisms are not fully understood. We used SMA-GFP transgenic mice to study myofibroblast recruitment and activation in skin wounds. Myofibroblasts were initially recruited to wounds three days post injury reached a maximum after seven days and subsequently declined. Expression profiling showed that 1749 genes were differentially expressed in sorted myofibroblasts from wounds seven days post injury. GO-Term analysis associated most of these genes with the extracellular region and cell periphery including upregulated genes encoding for ECM proteins, integrins and focal adhesion proteins and a specific set of growth factors, cytokines and chemokines promoting the healing response. In contrast, cell adhesion molecules involved in cell-cell interaction were downregulated. Novel genes not yet known to be expressed in myofibroblast were found. Hence, this first characterization of a myofibroblast specific expression network at the peak of granulation tissue formation in situ provides important insights into myofibroblast activation in skin wounds and identifies novel target genes to control excessive ECM deposition during fibrotic reactions.

2017-12-30 | GSE95786 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data