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Gene expression analysis of parental and sulfasalazine-resistant human oral squamous carcinoma cell line OSC19

ABSTRACT: To identify the genes involving in the resistance to xCT inhibitors, we established the sulfasalazine-resistant clone of an oral squamous cell carcinoma OSC19 cell line, and the resulting sulfasalazine-resistant cells and its parental cells were subjected to cDNA microarray analysis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE113860 | GEO | 2018/05/01

REPOSITORIES: GEO

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Gene expression analysis of parental and sulfasalazine-resistant human oral squamous carcinoma cell line OSC19

Project description:Gene expression analysis of parental and sulfasalazine-resistant human oral squamous carcinoma cell line OSC19

| PRJNA454284 | ENA

Effect of FOXA1 knockdown on gene expression in sulfasalazine-resistant OSC19SSZR cells

Project description:High-throughput sequencing was performed to analyze genes regulated by the transcription factor FOXA1 in OSC19SSZR cells, a sulfasalazine-resistant oral squamous cell carcinoma cell line.

2023-09-15 | GSE241108 | GEO

System Xc- exacerbates metabolic stress under glucose depletion in oral squamous cell carcinoma

Project description:<h4><strong>OBJECTIVE:</strong> Emerging evidence suggests that glucose depletion (GD)-induced cell death depends on system Xc-, a glutamate/cystine antiporter extensively studied in ferroptosis. However, the underlying mechanism remains debated. Our study confirmed the correlation between system Xc- and GD-induced cell death and provided a strategic treatment for oral squamous cell carcinoma (OSCC).</h4><p><strong>METHODS:</strong> qPCR and Western blotting were performed to detect changes in xCT and CD98 expression after glucose withdrawal. Then, the cell viability of OSCCs under the indicated conditions was measured. To identify the GD-responsible transcriptional factors of SLC7A11, we performed a luciferase reporter assay and a ChIP assay. Further, metabolomics was conducted to identify changes in metabolites. Finally, mitochondrial function and ATP production were evaluated using the seahorse assay, and NADP+/NADPH dynamics were measured using a NADP+/NADPH kit.</p><p><strong>RESULTS:</strong> In OSCCs, system Xc- promoted GD-induced cell death by increasing glutamate consumption, which promoted NADPH exhaustion and TCA blockade. Moreover, GD-induced xCT upregulation was governed by the p-eIF2α/ATF4 axis.</p><h4><strong>CONCLUSIONS:</strong> System Xc- overexpression compromised the metabolic flexibility of OSCC under GD conditions, and thus, glucose starvation therapy is effective for killing OSCC cells.</h4>

2024-01-16 | MTBLS5358 | MetaboLights

Expression data from fibroblasts derived from human normal oral mucosa, oral dysplasia and oral squamous cell carcinoma

Project description:Identification of genes that are differentially regulated in fibroblasts derived from dysplastic oral mucosa and oral squamous cell carcinoma compared to fibroblasts derived from normal oral mucosa. Affymetrix microarrays were used to define differential gene expression. Populations of fibroblasts were isolated from human normal oral mucosa, oral dysplasia and oral squamous cell carcinoma, maintained in 3D collagen I biomatrices, RNA extracted and processed for Affymetrix arrays. Fibroblasts maintained as monolayers were also included as comparators.

2013-12-01 | E-GEOD-38517 | biostudies-arrayexpress

Affymetrix SNP array data for oral squamous cell carcinoma and oral dysplasia samples

Project description:The development of oral squamous cell carcinoma (OSCC) is a multistep process requiring the accumulation of genetic alterations. Oral carcinogenesis is a multifactorial process involving numerous genetic changes that affect the activity of oncogenes, tumor suppressor genes and other classes of disease-related genes.Therefore, to identify the responsive genes for progression of oral dysplasia or OSCC, we here performed CGH analysis to DNA from oral dysplasia and OSCC by microdissection Copy number analysis of Affymetrix 250K SNP arrays was performed for 8 oral dysplasia samples, 8 oral squamous cell carcinoma samples, using microdissection

2013-10-22 | E-GEOD-51265 | biostudies-arrayexpress

Identification of CMTM6 as a novel mediator of cisplatin resistance in oral squamous cell carcinoma

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Expression data from fibroblasts derived from human normal oral mucosa, oral dysplasia and oral squamous cell carcinoma

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RNA-Seq of oral squamous cell carcinomas and matched normal tissues

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2010-02-25 | E-GEOD-20116 | biostudies-arrayexpress

Epigenome analysis of parental and ABT-199 resistant AML cell lines

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2022-01-12 | GSE193254 | GEO

Apolipoprotein E promotes invasion in oral squamous cell carcinoma

Project description:Genome-wide expression array measurements for 9 head and neck squamous cell carcinomas (HNSCC) stratified by worst pattern of invasion (WPOI) Jayakar et al. (2016). Apolipoprotein E promotes invasion in oral squamous cell carcinoma. Li et al. (2013). Validation of the risk model: high-risk classification and tumor pattern of invasion predict outcome for patients with low-stage oral cavity squamous cell carcinoma.

2016-04-01 | GSE79795 | GEO

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