Transcriptomics

Dataset Information

159

Transcriptional profiles of miR-155 overexpressing CD8T cells during chronic infection


ABSTRACT: Persistent viral infections and tumors drive development of exhausted T (TEX) cells. In these settings, TEX cells establish an important host-pathogen or host-tumor stalemate. However, TEX cells erode over time, leading to loss of pathogen or cancer containment. We identified microRNA (miR)-155 as a key regulator of sustained TEX cell responses during chronic lymphocytic choriomeningitis virus (LCMV) infection. Genetic deficiency of miR-155 ablated CD8 T cell responses during chronic infection. Conversely, enhanced miR-155 expression promoted expansion and long-term persistence of TEX cells. However, rather than strictly antagonizing exhaustion, miR-155 promoted a terminal TEX cell subset. Transcriptional profiling identified coordinated control of cell signaling and transcription factor pathways, including the key AP-1 family member Fosl2. Overexpression of Fosl2 reversed the miR-155 effects, identifying a link between miR-155 and the AP-1 transcriptional program in regulating TEX cells. Thus, we identify a mechanism of miR-155 regulation of TEX cells and a key role for Fosl2 in T cell exhaustion. Overall design: RNA-Seq analysis was used to investigate how miR-155 affects the expansion and differentiation of CD8 Tcells during chronic infection 12 samples from 4 groups, 3 replicates each

INSTRUMENT(S): Illumina HiSeq 1000 (Mus musculus)

ORGANISM(S): Mus musculus  

SUBMITTER: E JOHN WHERRY 

PROVIDER: GSE114482 | GEO | 2018-05-16

REPOSITORIES: GEO

altmetric image

Publications


Persistent viral infections and tumors drive development of exhausted T (TEX) cells. In these settings, TEX cells establish an important host-pathogen or host-tumor stalemate. However, TEX cells erode over time, leading to loss of pathogen or cancer containment. We identified microRNA (miR)-155 as a key regulator of sustained TEX cell responses during chronic lymphocytic choriomeningitis virus (LCMV) infection. Genetic deficiency of miR-155 ablated CD8 T cell responses during chronic infection.  ...[more]

Similar Datasets

| GSE10864 | GEO
2008-03-17 | E-GEOD-10864 | ArrayExpress
2014-09-04 | E-GEOD-58004 | ArrayExpress
| PRJNA471517 | ENA
| GSE10863 | GEO
| GSE79732 | GEO
2015-09-08 | E-GEOD-72752 | ArrayExpress
| GSE79731 | GEO
2012-04-03 | GSE29388 | GEO
| GSE72752 | GEO