Genome wide analysis of chick limb bud tissue treated with FGF2 for 3hrs (RNA-Seq)

ABSTRACT: During limb development, in addition to proximal to distal outgrowth, FGF regulates a number of factors necessary for SHH expression. To elucidate the molecules involved in FGF-mediated SHH expression we implanted FGF2 beads in HH23 chicken forelimb buds and excised the SHH-expressing tissue for transcriptome analysis. The hypothesis is that this transcriptome data along with data from SHH-expressing tissue after a 24hr treatment would highlight molecules necessary for SHH FGF-mediated SHH expression. Results from the overlap provide a short-list of SHH-related genes which require further testing to assess their role in mediating SHH expression.

ORGANISM(S): Gallus gallus

PROVIDER: GSE114662 | GEO | 2018/12/18

REPOSITORIES: GEO