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Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) sequencing of msh1 memory line

ABSTRACT: Col-0 wild type and msh1 memory line were grown, crosslinked and chromatin isolated as described in (Omidbakhshfard et al., 2014). Briefly, plants were germinated in MS media at 24°C, 12 hr light and 12 hr dark cycle for 14 days and crosslinked with formaldehyde. DNA was sonicated to get a size range of 250-800bp. Samples were divided equally into two lots and one was used directly for FAIRE experiments while the other lot was decrossed-linked and compared with the crosslinked samples to confirm he FAIRE treatment. FAIRE analysis was carried out on crosslinked sampes of wild ype and msh1 memory line.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE114664 | GEO | 2019/05/18

REPOSITORIES: GEO

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