Ribosome profilling and RNA Sequencing of WT and Rasgrp1Anaef mouse CD4+ T cells
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ABSTRACT: To determine what mRNAs are translated at baseline in CD4+ T cells, and whether these are altered in Rasgrp1Anaef CD4+ T cells, which have a point mutation in the Ras GEF Rasgrp1, before the onset of autoimmunity, we performed sequencing of ribosome-protected fragments as well as total RNA from WT and Anaef T cells. We identified a number of target genes that are translated at baseline, many of which have been shown to be downstream of the kinase mTOR.
ORGANISM(S): Mus musculus
PROVIDER: GSE114741 | GEO | 2019/05/07
REPOSITORIES: GEO
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