Genomics

Dataset Information

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Transcriptional profiling of cochlear neurons


ABSTRACT: In order to characterize the various subpopulations of neurons present in the inner ear, we dissected out the cochleas from wild-type mice, removing non-neuronal populations as possible, then dissociated the tissue to obtain a single cell suspension and profiled these by single cell RNA-Seq. Overall design: This study's goal was to characterize the native populations of murine cochlear neurons. To ensure reproducibility of our findings, neurons from 4 separate mice were profiled.

INSTRUMENT(S): Illumina NovaSeq 6000 (Mus musculus)

SUBMITTER: Gabriela Pregernig 

PROVIDER: GSE114759 | GEO | 2018-08-13

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE114759_RAW.tar Raw
GSE114759_SGNs_metadata.txt.gz Txt
GSE114759_SGNs_normCounts.txt.gz Txt
GSE114759_SGNs_rawCounts.txt.gz Txt
filelist.txt Txt
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Publications

Hair Cell Mechanotransduction Regulates Spontaneous Activity and Spiral Ganglion Subtype Specification in the Auditory System.

Sun Shuohao S   Babola Travis T   Pregernig Gabriela G   So Kathy S KS   Nguyen Matthew M   Su Shin-San M SM   Palermo Adam T AT   Bergles Dwight E DE   Burns Joseph C JC   Müller Ulrich U  

Cell 20180802 5


Type I spiral ganglion neurons (SGNs) transmit sound information from cochlear hair cells to the CNS. Using transcriptome analysis of thousands of single neurons, we demonstrate that murine type I SGNs consist of subclasses that are defined by the expression of subsets of transcription factors, cell adhesion molecules, ion channels, and neurotransmitter receptors. Subtype specification is initiated prior to the onset of hearing during the time period when auditory circuits mature. Gene mutations  ...[more]

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