Transcriptomics,Genomics

Dataset Information

31

The Hyphal Associated Adhesin and Invasin Als3 of Candida albicans Mediates Iron Acquisition from Host Ferritin


ABSTRACT: Iron sequestration by host iron-binding proteins is an important mechanism of resistance to microbial infections. Inside oral epithelial cells, iron is stored within the ferritin, and is therefore usually not accessible to pathogenic microbes. We observed that the ferritin concentration within oral epithelial cells was directly related to their susceptibility to damage by the human pathogenic fungus Candida albicans. Thus, we hypothesized that host ferritin may be used as an iron source by this organism. A screen of C. albicans mutants lacking components of each of the three iron acquisition systems revealed that only the reductive pathway is involved in iron utilization from ferritin by this organism. Transcriptional profiling of wild-type and hyphal-defective C. albicans strains suggested that the C. albicans invasin-like protein Als3 plays a role in ferritin binding. Overall design: Total RNA from wild-type (CAI4) and hyphal-defective C. albicans strains (deltaHGC1, deltaRAS1) was prepared after growth under iron-limiting conditions. Each strain was labeled and compared to a common control (C. albicans strain SC5314) in four independent experiments including two dye swaps.

INSTRUMENT(S): Eurogentec Candida albicans 6k microarray (condensed)

SUBMITTER: Bernhard Hube  

PROVIDER: GSE11490 | GEO | 2008-05-20

SECONDARY ACCESSION(S): PRJNA106421

REPOSITORIES: GEO

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Publications

the hyphal-associated adhesin and invasin Als3 of Candida albicans mediates iron acquisition from host ferritin.

Almeida Ricardo S RS   Brunke Sascha S   Albrecht Antje A   Thewes Sascha S   Laue Michael M   Edwards John E JE   Filler Scott G SG   Hube Bernhard B  

PLoS pathogens 20081121 11


Iron sequestration by host iron-binding proteins is an important mechanism of resistance to microbial infections. Inside oral epithelial cells, iron is stored within ferritin, and is therefore not usually accessible to pathogenic microbes. We observed that the ferritin concentration within oral epithelial cells was directly related to their susceptibility to damage by the human pathogenic fungus, Candida albicans. Thus, we hypothesized that host ferritin is used as an iron source by this organis  ...[more]

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