Nucleosomes stabilize ssRNA-dsDNA triple helices in human cells

ABSTRACT: The molecular mechanism for ssRNA+dsDNA triple helix structure formation on chromatin are unclear. We analyzed the triplex-nucleosomes complex fomration in vitro and in vivo, and show that triplexes are stabilized by the nucleosomes. We developed a method to monitor nucleosome bound RNA triplexes in vivo, revealing that RNA binding maintained the nucleosomes on an accessible structure supporting a gene activating role of nucleosome-triplex complexes in cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE114900 | GEO | 2019/02/18

REPOSITORIES: GEO