CRISPR/Cas9 genome editing demonstrates that CCM3 modulates endothelial miRNA expression

ABSTRACT: CCM3 regulates blood-brain-barrier integrity and vascular maturation in vivo. CCM3 loss-of-function variants predispose to cerebral cavernous malformations (CCM). Various signalling pathways are deregulated upon CCM3 depletion in endothelial cells (ECs). In this study, we established a crRNA:tracrRNA:Cas9 RNP approach to efficiently knockout CCM3 in human ECs and studied the molecular and functional effects of its long-term inactivation. Using small RNA sequencing, we show that CCM3 regulates the expression of aging‑associated miRNAs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE114947 | GEO | 2019/01/18

REPOSITORIES: GEO