Single cell RNA-Seq analysis provide deeper molecular insights into abnormal maturation of tubastatin A-treated mouse oocytes

ABSTRACT: In our previous study, we observed that Tub-A treatment induced an increased level of the acetylation of alpha-tubulin, and a failure of spindle migration and actin cap formation. Most Tub-A treated oocytes were arrested in an MI-like or a GVBD-like stage and decreased mTOR (spindle formation factor) and mDia1 (inhibitor of actin assembly) in an HDAC6 expression-dependent manner. We wonder if TubA only affects HDAC6 expression. Therefore the purpose of this study is to clarify the cause of the phenomenon through RNA-seq analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE115008 | GEO | 2018/11/30

REPOSITORIES: GEO