Culture on stiff matrix changes the whole-genome transcriptional profile of fibroblasts

ABSTRACT: The behavior of cells is strongly determined by the stiffness of extracellular matrix. In lung fibrosis, stiff lung tissue promotes the transformation of myofibroblasts, which secrect an excess of extracellular matrix proteins, and play an central role in the progression of fibrosis. To screen which transcripts were involved in stiff matrix induced myofibroblasts transformation, mouse fibroblasts (NIH3T3) cultured on different stiffness (1 KPa and 4 KPa) hydrogels coated with rat tail type I collagen for 48h, then the whole -genome transcriptional profiles of fibroblasts were analyzed. Of the 26,423 detected transcripts, 170 were significantly upregulated and 98 were significantly downregulated (p<0.05) in fibroblasts cultured on stiff matrix. KEGG pathway enrichment analyses found that ECM-receptor interaction and Focal adhesion were the first two significantly enriched pathway. Overall design: Fibroblasts were cultured on soft and stiff matrix for 48h, and then cells were collected for microarray experiments.

INSTRUMENT(S): Phalanx Mouse OneArray Ver 2

ORGANISM(S): Mus Musculus

SUBMITTER: CHUN CHEN 

PROVIDER: GSE115260 | GEO | 2018-06-05

REPOSITORIES: GEO