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Identification of novel ETn insertions in KRAB-ZFP cluster depleted mice

ABSTRACT: Genetic deletion of a large KRAB-ZFP gene cluster on chromosome 4 (Chr4-cl) leads to transcriptional reactivation of retrotransposons (mainly ETn elements) in ES cells. We therefore wanted to test whether ETn elements retrotranspose in Chr4-cl KO mice at higher frequency. For this we purified genomic DNA from WT and KO mice, enriched gDNA libraries using ETn capture probes and sequenced those libraries as paire-end reads. These reads were then used to identify novel ETn insertions that are not annotated in the reference genome or present in the germ line of their progenitors.

ORGANISM(S): Mus musculus

PROVIDER: GSE115285 | GEO | 2019/06/27

REPOSITORIES: GEO

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