Epigenetic optical mapping of 5-hydroxymethylcytosine in nanochannel arrays

ABSTRACT: We present a long-read, single-molecule mapping technology that generates hybrid genetic/epigenetic profiles of native chromosomal DNA. The genome-wide distribution of 5-hmC in human peripheral blood cells correlates well with 5-hmC DNA immunoprecipitation (hMeDIP) sequencing. However, the long single-molecule read-length of 100 kbp-1 Mbp produces 5-hmC profiles across variable genomic regions that failed to show up in the sequencing data. In addition, optical 5-hmC mapping shows a strong correlation between the 5-hmC density in gene bodies and the corresponding level of gene expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE115454 | GEO | 2018/06/08

REPOSITORIES: GEO