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HiChIRP reveals RNA-associated chromosome conformation

ABSTRACT: Modular domains of lncRNAs can serve as scaffolds to bring distant regions of the linear genome into spatial proximity. Here we present HiChIRP, a method leveraging novel bioorthogonal chemistry and customized 3C conditions, that enables interrogation of chromatin architecture focused around a specific RNA of interest down to ~10 copies per cell. HiChIRP of three nuclear RNAs reveal insights into promoter interactions (7SK), telomere biology (TERC), and inflammatory gene regulation (lincRNA-EPS).

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE115524 | GEO | 2018/12/04

REPOSITORIES: GEO

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A long noncoding RNA lincRNA-EPS acts as a transcriptional brake to restrain inflammation

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