Comparison of liver mRNA expression between Control AA and Low AA diet fed mice
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE115683 | GEO | 2019/01/17
REPOSITORIES: GEO
Similar Datasets
Project description:Dietary proteins have profound effects on lipid metabolism but the mechanism remains to be elucidated. In the present study, we examined the temporal impact of dietary proteins in isoenergetic high fat diets on lipid metabolism of C57BL/6J mice. Mice were first fed a low protein (P) to carbohydrate (C) ratio high-fat diet (L-P/C-HF) for 10 weeks and then a half of mice were changed to a high protein to carbohydrate ratio high-fat diet (H-P/C-HF) for additional 4 weeks whereas the remaining mice continued eating the L-P/C-HF diet.
2012-12-19 | E-GEOD-20260 | biostudies-arrayexpress
Project description:Carbohydrate response element binding protein (ChREBP) is one of the major transcription factors regulating carbohydrate metabolism and lipogenesis.It expresses highly in several tissues including liver, adipose tissue, small intestine,kidney and muscles. Mice with global knockout of ChREBP exhibit intolerance to carbohydrate including glucose and fructose. However, the exact role of liver ChREBP in high carbohydrate stress is not well defined. We used microarrays to exame the changes of gene expression pfofile upon high sucrose (50% glucose and 50% fructose) stress when liver ChREBP was deleted.
2020-01-24 | GSE139937 | GEO
Project description:To elucidate the effects of altered dietary carbohydrate and fat balance on liver and adipose tissue transcriptomes, 3-week-old rats were fed three kind of diets: low, moderate and high fat diets (L, M and H) containing a different ratio of carbohydrate:fat (C:F) (65:15, 60:20 and 35:45 in energy percent, respectively).
2017-03-24 | GSE79867 | GEO
Project description:Food is a fundamental requirement for organisms to develop, survive and reproduce. However, access to an optimum diet is not always guaranteed, resulting in a mismatch between the diet and the organism's needs. To comprehend the consequences of this mismatch, it becomes crucial to explore how different compositions of an organism's diet impact its overall performance. In this context, we tested the effect of isocaloric diets with different protein to carbohydrate ratios on eight key life history traits in a large (n = ~2,100) outbred Drosophila melanogaster population. We report accelerated pre-adult development and increased reproductive output, without affecting pre-adult viability and body size, in flies fed on low carbohydrate diet. We also observe that high carbohydrate content in diet shortens fly lifespan. In support, we found that increasing carbohydrate content in food led to faster aging by impairing locomotor activity and gut integrity. Additionally, we noted that the composition of the diet had an impact on gene expression patterns with high carbohydrate diets upregulating genes and high protein diets downregulating them. These results collectively provide a comprehensive understanding of how the balance of macronutrients in an organism's diet intricately shapes its traits.
2024-02-14 | GSE241397 | GEO
Project description:The tongue is a heavily innervated and vascularized striated muscle that plays an important role in vocalization and swallowing. The surface of the tongue is lined with papillae which contain gustatory cells expressing various taste receptors. There is growing evidence to suggest our perceptions of taste and food preference are remodelled following chronic consumption of western diets rich in carbohydrate and fats. Our sensitivity to taste and also metabolism western diets may be a key factor in the rising prevalence of obesity but a systems-wide analysis is lacking. Here, we defined the proteomic landscape of the mouse tongue and quantified changes following chronic consumption of a chow or western diet for 7 months. We observed a dramatic remodelling of the tongue proteome including changes in fatty acid and mitochondrial metabolism. The expression of several receptors, and metabolic enzymes and hormones were also differentially regulated which is exciting as these may serve as novel therapeutic targets to potentially alter taste perception and food preference to combat obesity.
2021-09-10 | PXD025190 | Pride
Project description:The composition of the diet affects many processes in the body, including body weight and endocrine system. We have previously shown that dietary fat also affects the immune system. Mice fed high fat diet rich in polyunsaturated fatty acids survive S. aureus infection to a much greater extent than mice fed high fat diet rich in saturated fatty acids. Here we present data regarding the dietary effects on protein expression in spleen from mice fed three different diets, I) low fat/chow diet (LFD, n=4), II) high fat diet rich in saturated fatty acids (HFD-S, n=4) and III) high fat diet rich in polyunsaturated fatty acids (HFD-P, n=4). We performed mass spectrophotometry based quantitative proteomics analysis of isolated spleen by implementing the isobaric tags for relative and absolute quantification (iTRAQ) approach. Mass spectrometry data were analysed using Proteome Discoverer 2.4 software using the search engine mascot against Mus musculus in SwissProt. 924 proteins are identified in all sets (n=4) for different dietary effects taken for statistical analysis using Qlucore Omics Explorer software. Only 20 proteins were found to be differentially expressed with a cut-off value of false discovery rate < 0.1 (q-value) when comparing HFD-S and HFD-P but no differentially expressed proteins were found when LFD was compared with HFD-P or HFD-S. We identified a subset of proteins that showed an inverse expression pattern between two high fat diets. These differentially expressed proteins were further classified by gene ontology for their role in biological processes and molecular functions.
2020-08-06 | PXD020365 | Pride
Project description:Quercetin is a food component that may ameliorate the diabetic symptoms. We examined hepatic gene expression of BALB/c mice with streptozotocin (STZ)-induced diabetes to elucidate the mechanism of the protective effect of dietary quercetin on diabetes-associated liver injury. We fed STZ-induced diabetic mice with diets containing 0.1% or 0.5% quercetin for 2 weeks and compared the patterns of hepatic gene expression in these groups of mice using a DNA microarray. Diets containing 0.1% or 0.5% quercetin lowered the STZ-induced increase in blood glucose levels and improved plasma insulin levels. A cluster analysis of the hepatic gene expressions showed that 0.5% quercetin diet suppressed STZ-induced alteration of gene expression. Gene set enrichment analysis (GSEA) and quantitative RT-PCR analysis showed that the quercetin diets had their greatest suppressive effect on the STZ-induced elevation of expression of cyclin dependent kinase inhibitor p21(WAF1/Cip1) (Cdkn1a).
2012-05-23 | GSE38067 | GEO
Project description:Transcriptomic analysis of Streptococcus pneumoniae TIGR4 wildtype and it's isogenic ccpA mutant, grown in chemically defined media supplemented with physiological levels of carbohydrates to mimic the human nasopharynx and bloodstream. The goal is to examine how anatomical site-specific carbohydrate availability impacts S. pneumoniae physiology and virulence.
2021-11-18 | GSE189057 | GEO
Project description:Wistar Kyoto, inbred male rats (M & B, Ry, Denmark) were randomized and fed a semisynthetic pellet diet deficient in folate (TD350, Harland, Madison, Wisconsin, USA) or an identical diet with a normal amount of folate (TD351, Harland). Special analyses were performed to establish the content of Methionine, Vitamin B12, Folate and Vitamin B6 in the two diets. The folate content was <0.06 mg/kg and 1.63 mg/Kg, respectively. Detailed specification of the diets has been published previously. Animals were housed in stainless steel cages with a bed of sawdust, two to a cage, and were allowed free access to food and drinking water. The room temperature was 23a C and the humidity was 45-55 %. Light cycles consisted of 12 h light/12 h darkness. The animals were weighed at randomization and subsequently every seven days. The animal experiments were carried out in accordance with the guidelines of the International Scientific Committee for Thrombosis and Hemostasis, and the Danish Ethical Committee for Animal Experimentation had approved the study protocol. This study consisted of twelve rats fed the folate deficient diet and twelve rats fed the normal diet for 28 days.
2005-08-01 | E-MEXP-154 | biostudies-arrayexpress
Project description:To investigate the role of hepatic Pck1 in diet-induced nonalcoholic steatohepatitis (NASH), liver-specific Pck1-knockout mice were developed with Alb-Cre. Wild-type (WT) and Pck1-cKO mice were fed a Chow diet (Research Diets, D12450J: 10% Kcal fat, with tap water) or NASH-inducing diet (Research Diets, D12492: 60% Kcal fat, with drinking water containig 23.1 g/L fructose and 18.9 g/L glucose) for 24 weeks. RNA was extracted from the livers of mice from all treatment groups and used for RNA seqencing. RNA-seq data demonstrated that gluconeogenic enzyme PCK1 deficiency played an important role in the development of NASH.
2023-02-07 | GSE162211 | GEO