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Transcriptional analysis of adipose tissue during post-natal development reveals depot-specific responsiveness to maternal dietary supplementation

ABSTRACT: In this study we use ovis aries as a comparative model The objective of the study was to compared changes in genetic interactions in five adipose tissue deposits during early development.

ORGANISM(S): Ovis aries Homo sapiens

PROVIDER: GSE115799 | GEO | 2018/06/15

REPOSITORIES: GEO

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Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation.

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Transcriptional analysis of adipose tissue during post-natal development reveals depot-specific responsiveness to maternal dietary supplementation

Project description:Transcriptional analysis of adipose tissue during post-natal development reveals depot-specific responsiveness to maternal dietary supplementation

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Differential proteome between ejaculate and epididymal sperm represents a key factor for sperm freezability in wild small ruminants

Project description:The proteome of two sperm sources, epididymal and ejaculated sperm, was compared and associated with sperm freezing capacity using samples collected from three wild small ruminants species. Protein identification was performed using two databases separately (Ovis aries and Capra hircus NCBI) and results were later combined.

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Differential expression analysis of miRNAs in goat (Capra hircus) mammary gland between peak and late lactation by Solexa sequencing

Project description:We report the application of Solexa high-throuthput sequencing technology for miRNA differential expression profiles. Using an optimized data analysis workflow, we mapped about 30 million sequence reads per sample to Ovis aries genome. The results were validated by qRT-PCR. Our study represents the first detailed analysis of miRNA expression profiles in goat mammary gland tissues, with biologic replicates generated by RNA-seq technology.

2013-01-01 | GSE39484 | GEO

scRNA-seq of sheep abomasal epithelial tissue post-nematode infection

Project description:Tuft cells are required for an effective immune response to gastrointestinal parasitic nematodes in mice, but little is known about tuft cells in other animals. Using scRNA-seq, we profiled the transcriptome of tuft cells and other mucosal cell types from the sheep abomasal epithelium following nematode infection. Cells were isolated from abomasum mucosal tissue of two sheep and processed using 10x Genomics Chromium Single Cell 3’ Reagent kit (v.3) using a total of 20,000 input cells. cDNA libraries were sequenced on an Illumina NextSeq 500 system to a depth of 50,000 read pairs per cell. Sequences were mapped to the genomes for ovine (Ovis aries Oar_v3.1; https://www.ensembl.org/Ovis_aries/Info/Index) and bovine (Bos taurus ARS-UCD1.2; https://www.ensembl.org/Bos_taurus/Info/Index) and gene count matrices generated using the Cell Ranger software (v.3.1.0) and analysed using Seurat package (v.3.1) with R version 3.6.0. The “Find Markers” function identified a tuft cell cluster and other distinct epithelial (Epcam+) and immune (Ptprc+) cell clusters.

2021-11-16 | E-MTAB-10231 | biostudies-arrayexpress

liver

Project description:Ovis aries

| PRJNA791008 | ENA

liver Raw sequence reads

Project description:ovis aries

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Ovis aries breed:Suffolk-derived crossbred

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Aristaeus chip 1.0: an in situ synthesized oligonucleotide microarray for Ovis aries

Project description:Here we present a high-density in situ synthesized microarray for Ovis aries, named Aristaeus, designed by means of a pipeline of software instruments that, starting from non-annotated redundant EST sequences, selects oligonucleotides suitable for in situ generation on chip. The chip was tested by comparing the gene expression profiles of two sheep breeds with different phenotype, Sarda and Gentile di Puglia. We carried out microarray experiments on liver and udder tissues from lactating individuals and identified a relevant number of differentially expressed genes, all involved in metabolism pathways. The results are consistent with literature knowledge, while selected differential gene expressions have been confirmed by quantitative real-time polymerase chain reaction analyses. Tissue samples of liver were collected from 4 lactating individuals of two sheep (Ovis aries) breeds, Gentile di Puglia and Sarda. Biopsies of liver tissue were taken at second lactation stage (first record, stage 01: 6 days after lambing; second record, stage 02: 44 days after lambing) in both breeds. Tissues from liver were immersed in RNAlater (Sigma) immediately after biopsy and stored at -20°C. Samples were pooled by breed and then reverse labeled (cy5 and cy3), resulting in four raw data sets.

2013-02-05 | E-GEOD-31044 | biostudies-arrayexpress

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