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Real-time quantitative PCR analysis of induced pluripotent derived endothelial cells

ABSTRACT: CD31+/VECad+ hiPSC-ECs were purified through magnetic activated cell sorting. Expanded endothelial cells were then subjected to serum starvation conditions for 24 hours by culturing in DMEM. After serum starvation, total RNA was extracted. We used a custom Thermo Fisher Scientific PCR assay panel to quantify gene expression relevant to ciliogenesis, cytoskeletal integrity, Wnt signaling, pluripotency and endothelial phenotype.

ORGANISM(S): Homo sapiens

PROVIDER: GSE115870 | GEO | 2018/06/16

REPOSITORIES: GEO

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Project description:ARPE-19 RPE cells obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) were cultured in DMEM medium with 10% FBS (Thermo Fisher Scientific, Waltham, USA) at the incubator with 37 °C, 5% CO2 and 100% humidity. Final concentration of 15 μM curcumin (C7727, Sigma-Aldrich, St. Louis, USA) and 100 μM CoCl4 (10007216, Sinopharm Chemical Reagent, Shanghai, China) were added within serum-free medium for 24 h and 4 h respectively. RNA-seq was performed to investigate the transcriptome alteration.

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