A global map of RNA binding protein occupancy guides functional dissection of post-transcriptional regulation of the T cell transcriptome [Hs]
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ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE115886 | GEO | 2018/07/01
REPOSITORIES: GEO
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Project description:RBP binding sites were globally mapped by a biochemical technique in mouse and human T cells
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Project description:A global map of RNA binding protein occupancy guides functional dissection of post-transcriptional regulation of the T cell transcriptome [Hs]
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Project description:Gene expression programs depend on sequence-specific DNA binding transcription factors, but the mechanisms that control the selective binding of these factors in a chromosomal and genomic context remain enigmatic. Here, we show that two master regulators of B-cell fate, namely EBF1 and RBP-jk, show variable genome-wide chromosome distribution in two related B-lymphocyte lines carrying different forms of Epstein-Barr Virus (EBV) latency. The latency-type specific EBV-encoded EBNA2 colocalized with RBP-jk and EBF1 at induced binding sites. Colocalization of EBF1, RBP-jk, and EBNA2 correlated with transcriptional activation. Conditional expression or repression of EBNA2 lead to a rapid alteration in RBP-jk and EBF1 binding. Biochemical and shRNA depletion studies provide evidence for cooperative assembly at co-occupied sites. These findings reveal that non-DNA binding cofactors can facilitate combinatorial interactions to induce new patterns of transcription factor occupancy and gene programming
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