Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Immunological properties of neural crest cells derived from human induced pluripotent stem cells (normal vs inflammed condition)

ABSTRACT: Collecting primary neural crest cells (NCCs) of a sufficient quantity for some experiments is difficult, because NCCs are embryonic transient tissue and basically they do not proliferate. We successfully induced NCCs from human induced pluripotent stem cells (iPSCs) according to the previously described method with some modifications. iPSCs-derived NCCs (iPSC-NCCs) are reported to have potential for wound healing and expected to be used clinically in the future. However, nobody reported the immunological properties of iPSC-NCCs. It is important to evaluate the immunological properties of iPSC-NCCs before clinical use. In our previous study, we found that iPSC-NCCs have immunosuppressive properties in resting state. In the next step, we examined gene expression patterns between iPSC-NCCs (normal condition) and iPSC-NCCs (inflamed condition).

ORGANISM(S): Homo sapiens

PROVIDER: GSE116110 | GEO | 2018/12/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Immunological properties of neural crest cells derived from human induced pluripotent stem cells (resting state)

Project description:Collecting primary neural crest cells (NCCs) of a sufficient quantity for some experiments is difficult, because NCCs are embryonic transient tissue and basically they do not proliferate. We successfully induced NCCs from human induced pluripotent stem cells (iPSCs) according to the previously described method with some modifications. iPSCs-derived NCCs (iPSC-NCCs) are reported to have potential for wound healing and expected to be used clinically in the future. However, nobody reported the immunological properties of iPSC-NCCs. It is important to evaluate the immunological properties of iPSC-NCCs before clinical use. We examined gene expression patterns between iPSCs and iPSC-NCCs.

2018-12-01 | GSE109136 | GEO

Immunological properties of retinal ganglion cells derived from human induced pluripotent cells

Project description:In recent years, research has been conducted to develop retinal ganglion cells (RGCs) transplantation therapies. Although rejection is one of the problems with transplantation therapy, the immunological properties of RGCs have not been clarified. We successfully induced RGCs from human induced pluripotent stem cells (iPSCs). We examined the gene expression patterns of iPS-RGCs using iPSC (a same donor) and peripheral mononuclear blood cells (PBMCs: a same donor) as a control.

2020-10-31 | GSE154544 | GEO

CHARGE syndrome modeling using patient-derived iPSC reveals defective migration of neural crest cells harboring CHD7 mutations

Project description:CHARGE syndrome is caused by heterozygous mutations in a chromatin remodeler CHD7 and characterized by a set of malformations historically postulated to arise from defects in the neural crest formation during embryogenesis. To better delineate neural crest defects in CHARGE syndrome, we generated induced pluripotent stem cells (iPSCs) from two patients with typical syndrome manifestations, and characterized neural crest cells differentiated in vitro from these iPSCs (iPSC-NCCs). We found that expression of genes associated with cell migration was altered in CHARGE iPSC-NCCs as compared to control iPSC-NCCs. Consistently, CHARGE iPSC-NCCs showed defective delamination, migration and motility in vitro, and their transplantation in ovo revealed overall defective migratory activity in the chick embryo. Altogether, our results support the historical inference that CHARGE syndrome patients have defects in neural crest migration and provide the first successful application of patient-derived iPSCs in modeling craniofacial disorders.

2017-10-23 | GSE86212 | GEO

Time course expression pattern during neural crest cells (NCCs) differentiation from iPSCs

Project description:To clarify the changes in cell properties during the differentiation process from iPSCs into NCCs, we sampled each cell during the differentiation process and compared gene expression patterns.

2022-06-18 | GSE206048 | GEO

Generation of mesenchymal stem/stromal cells (MSCs) from human iPSCs via neural crest cells (NCCs)

Project description:To clarify the changes in cell properties during the differentiation process from iPSCs into MSC through NCCs, we sampled each cell during the differentiation process and compared gene expression patterns.

2022-06-19 | GSE206128 | GEO

Derivation and characterization of putative craniofacial mesenchymal stromal cells from human induced pluripotent stem cells

Project description:The introduction and widespread adoption of induced pluripotent stem cell (iPSC) technology has opened new avenues for craniofacial regenerative medicine. Neural crest cells (NCCs) are the precursor population to many craniofacial structures, including dental and periodontal structures, and iPSC-derived NCCs may, in the near future, offer an unlimited supply of patient-specific cells for craniofacial repair interventions. Here, we used an established protocol involving simultaneous Wnt signaling activation and TGF-β signaling inhibition to differentiate three human iPSC lines to cranial NCCs. We then derived a putative craniofacial mesenchymal progenitor (PCMP) population with chondrogenic and osteogenic potential from cranial NCCs and investigated their similarity to widely studied human postnatal dental or periodontal stem/progenitor cells. PCMPs were quite distinct from both their precursor cells (NCCs) and bone-marrow mesenchymal stromal cells, a stromal population of mesodermal origin. Despite their similarity with dental stem/progenitor cells, PCMPS were clearly differentiated by a core set of 66 genes, including ACKR3 (CXCR7), whose expression (both at transcript and protein level) appear to be peculiar to PCMPs. Altogether, our data demonstrate the feasibility of craniofacial mesenchymal progenitor derivation from human iPSCs through a neural crest-intermediate and set the foundation for future studies regarding their full differentiation repertoire and their in vivo existence.

2018-10-22 | GSE113297 | GEO

Expression data from neural crest cells and neural crest cell-derived MSCs from human pluripotent stem cells of FOP patients and controls

Project description:NCCs and NCC-derived MSCs were induced from FOP-iPSCs and control iPSCs, and their expresion profiles were compared. Comparison of gene expressions among hiPSCs, hESCs, hNCCs and hNC-MSCs from FOP-iPSCs and control-iPSCs.

2015-09-17 | E-GEOD-61510 | biostudies-arrayexpress

Human induced pluripotent stem cells: diploid, chr7q deletion, spontaneous chr7 correction

Project description:Gene expression analysis, a) comparing isogenic karyotypically normal iPSCs to del7q-iPSCs, b) comparing del7q-iPSCs to spontaneously corrected iPSCs. The chr7q deletion results in reduced expression levels of a large number of genes in the chr7q deleted region Two-condition experiment, del7q-iPSCs vs. isogenic normal iPSCs and del7q-iPSCs vs spontaneously corrected-iPSCs. Biological replicates: 3 control replicates, 3 del7q-iPSC replicates and 3 spontaneously corrected-iPSC replicates

2015-02-16 | E-GEOD-65215 | biostudies-arrayexpress

Immunological properties of neural crest cells derived from human induced pluripotent stem cells (normal vs inflammed condition)

Project description:Immunological properties of neural crest cells derived from human induced pluripotent stem cells (normal vs inflammed condition)

| PRJNA477291 | ENA

Human induced Pluripotent stem cells: Normal diploid line vs lines with deletions of Chr7

Project description:Mapping deletions of Chr7 in human iPSC derived from patients cells through reprogramming Two-condition experiment, normal diploid iPSCs vs del7q-iPSCs

2015-02-16 | E-GEOD-65387 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data