Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome of day 6 EBs [WT, hWDR5iRescue,Wdr5 KO clone #3 with or without early (T12h) and late rescue (T48h)]

ABSTRACT: This study describes the transcriptome profiling of day 6 SFEBq embryonic bodies (EBs): 1) WT; 2) Wdr5 KO ; 3) Wdr5 KO with T12h hWDR5 rescue; 4) Wdr5 KO with T48h hWDR5 rescue

ORGANISM(S): Mus musculus

PROVIDER: GSE116155 | GEO | 2019/11/21

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome of Wdr5 and (or) p53 double knockout ESCs and embryoid bodies

Project description:This study describes the transcriptome profiling of day 6 SFEBq embryonic bodies (EBs): 1) WT; 2) Wdr5 KO ; 3) Wdr5 KO with T12h hWDR5 rescue; 4) Wdr5 KO with T48h hWDR5 rescue

2021-06-22 | GSE178555 | GEO

Chromatin accessibility profiling of mouse embryonic stem cells and embryonic bodies using SFEBq differentiation methods upon Wdr5 and P53 deletion with or without WT or mutant hWDR5 rescue

Project description:This study describes time-course chromatin accessibility profiling of mouse ESC and embryonic bodies n Wdr5 and p53 knockout (with or without WT or mutant hWDR5 rescue)

2021-06-22 | GSE178551 | GEO

Transcriptome of day 6 EBs [WT, hWDR5iRescue,Wdr5 KO clone #3 with or without early (T12h) and late rescue (T48h)]

Project description:Transcriptome of day 6 EBs [WT, hWDR5iRescue,Wdr5 KO clone #3 with or without early (T12h) and late rescue (T48h)]

| PRJNA477503 | ENA

Chromatin accessibility profiling of mouse embryonic bodies using SFEBq differetiation methods upon transient mWdr5 deletion with or without hWDR5 rescue

Project description:This study describes time-course chromatin accessibility profiling of mouse embryonic bodies upon transient mWdr5 deletion with or without hWDR5 rescue

2019-11-21 | GSE116153 | GEO

RNA Sequencing of H1 WT hESCs, H1 QSER1 KO hESCs, H1 TET1 KO hESCs, H1 QSER1/TET1 DKO hESCs, WT Day10 EBs, QSER1 KO Day10 EBs, TET1 KO Day10 EBs, QSER1/TET1 DKO Day10 EBs, WT PP1, QSER1 KO PP1, TET1 KO PP1, and QSER1/TET1 DKO PP1.

Project description:RNA Sequencing of H1 WT hESCs, H1 QSER1 KO hESCs, H1 TET1 KO hESCs, H1 QSER1/TET1 DKO hESCs, WT Day10 embryoid bodies (EBs), QSER1 KO Day10 EBs, TET1 KO Day10 EBs, QSER1/TET1 DKO Day10 EBs, WT pancreatic progenitors (PP1), QSER1 KO PP1, TET1 KO PP1, and QSER1/TET1 DKO PP1. DNA methylation is essential to mammalian development, and dysregulation can cause serious pathological conditions. Key enzymes responsible for deposition and removal of DNA methylation are known, but how they cooperate to tightly regulate the methylation landscape remains a central question. Utilizing a knockin DNA methylation reporter, we performed a genome-wide CRISPR/Cas screen in human embryonic stem cells to discover DNA methylation regulators. The top screen hit was an uncharacterized gene QSER1, which proved to be a key guardian of bivalent promoters and poised enhancers of developmental genes, especially those residing in DNA methylation valleys (or canyons). We further demonstrate cooperation of QSER1 and TET1 through genetic and biochemical interactions to inhibit DNMT3-mediated de novo methylation and safeguard developmental programs.

2021-03-19 | GSE169209 | GEO

Chromatin accessibility profiling of mouse embryonic stem cells and embryonic bodies using SFEBq differentiation methods upon Wdr5 and P53 deletion with or without WT or mutant hWDR5 rescue

Project description:Chromatin accessibility profiling of mouse embryonic stem cells and embryonic bodies using SFEBq differentiation methods upon Wdr5 and P53 deletion with or without WT or mutant hWDR5 rescue

| PRJNA739652 | ENA

Wdr5

Project description:Wdr5

| PRJNA739648 | ENA

Genome profiling of WDR5, P53, H3K4Me3 binding in mouse embryonic bodies using SFEBq differetiation methods

Project description:This study describes the binding profile of WDR5, p53 and H3K4Me3 in mouse embryonic bodies at day 2 (WT, Wdr5 KO, Wdr5 and p53 double knockout)

2019-11-25 | GSE140899 | GEO

Genome profiling of HA-hWDR5 binding in mouse embryonic bodies using SFEBq differetiation methods

Project description:This study describes the binding profile of HA-hWDR5 in mouse embryonic bodies with early (T12h) and late (T48h) rescue of HA-hWDR5

2019-11-21 | GSE116154 | GEO

Altered expression of genes in WDR5 knockdown

Project description:WDR5 (WD repeat domain 5) plays an important role in the epigenetic regulation of gene transcription, involing in regulation of embryonic stem cell and cancer cell.Bladder cancer is one of the most common cancers that cause approximately 150,000 deaths per year worldwide.The goal of this study is to identify the target genes of WDR5 in bladder cancer.Our results inditcate that the genes regulated by WDR5 involved in a variety of biological functions, such as proliferation and anti-apoptosis. Bladder cancer cell line UM-UC-3 was transfected with the contol siRNA or two different WDR5 siRNA for 48h. The RNA of the transfected cells was extracted and hybridized on Affymetrix microarrays. We indentified the putative target genes of WDR5 in both WDR5 siRNA by comparing the diferent expressed genes among control, si-WDR5-2 and si-WDR5-3. We futher validated the data of microarray in UM-UC-3 and T24 by RT-qPCR and Western bloting.

2015-02-09 | E-GEOD-59132 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data