Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome analysis of EDS1-R493A after Pst AvrRps4- triggered immunity

ABSTRACT: We present here the transcriptominc changes occuring after TNL-triggered immunity after infection with pst AvrRps4 in a point mutant of EDS1, EDS1-R493A

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE116269 | GEO | 2019/02/26

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome analysis of EDS1 4h after Pst AvrRps4- triggered immunity

Project description:We present here the transcriptominc changes occuring after TNL-triggered immunity after infection with pst AvrRps4 in a point mutant of EDS1, EDS1-R493A. This data of 4 h treatment, adds upon the previous study GSE116269.

2021-04-01 | GSE148304 | GEO

Transcriptome analysis of TPR1/TPL mutant lines during Pst AvrRps4- triggered immunity

Project description:Plant Topless (TPL) and Topless-related (TPR) corepressors are important regulators of plant hormone and immunity signaling. We present here the transcriptome of tpr1 and tpr1 tpl tpr4 triple mutant lines (t3) during TNL-triggered immunity in Arabidopsis thaliana after infection with Pst avrRps4 (OD 0.005). Col-0 and eds1-2 were analysed as controls.

2021-06-29 | GSE154774 | GEO

Transcriptome analysis of EDS1 4h after Pst AvrRps4- triggered immunity

Project description:Transcriptome analysis of EDS1 4h after Pst AvrRps4- triggered immunity

| PRJNA623859 | ENA

Transcriptome analysis of EDS1-R493A after Pst AvrRps4- triggered immunity

Project description:Transcriptome analysis of EDS1-R493A after Pst AvrRps4- triggered immunity

| PRJNA477936 | ENA

Expression analysis of EDS1-NLS

Project description:TIR-type nucleotide-binding leucine-rich repeat domain proteins (TNLs) constitute one major group of immune receptors in dicotyledonous plants. Under normal conditions, TNLs can detect non-self or modified-self within the plant cytoplasm to activate immune signaling characterized by extensive transcriptional reprogramming and efficiently counteracting pathogen infection. At the same time, TNLs, in negative epistatic interaction with a second endogeneous locus or allele are causal for induction of autoimmunity or hybrid necrosis. Both native, pathogen-induced TNL responses and autoimmunity are fully dependent on the plant-specific lipase-like protein EDS1, which is a central integrator for all TNL-mediated responses. EDS1 signals within structurally similar, but spatially distinct complexes with PAD4 and SAG101. We here analyzed stable transgenic lines expressing an EDS1 fusion with enforced nuclear localization. Even in absence of SAG101, nuclear-localized EDS1-PAD4 complexes are fully sufficient to function in basal and effector-triggered immunity. Furthermore, we show that nuclear EDS1, when expressed to high levels, can induce autoimmuity in combination with an RPP1-like gene cluster from ecotype Ler. RPP1-like genes are also implicated in several cases of hybrid necrosis, and we can identify the RPP1 paralog R8 as causal for autoimmunity induction by nuclear EDS1 and a previously characterized, EMS-induced mutation. This highlights the important role of EDS1-family proteins in the nuclear compartment in different immune-like responses.

2017-02-09 | GSE65415 | GEO

Transcriptome analysis of TPR1/TPL mutant lines during Pst AvrRps4- triggered immunity

Project description:Transcriptome analysis of TPR1/TPL mutant lines during Pst AvrRps4- triggered immunity

| PRJNA647408 | ENA

The EDS1-PAD4-ADR1 node mediates Arabidopsis pattern-triggered immunity

Project description:Plants deploy cell surface and intracellular leucine rich-repeat domain (LRR) immune receptors to detect pathogens. LRR receptor kinases (LRR-RKs) and LRR receptor proteins (LRR-RPs) recognise microbe-derived molecules to elicit pattern-triggered immunity (PTI), whereas nucleotide-binding LRR (NLR) proteins detect microbial effectors inside cells to confer effector-triggered immunity (ETI). Although PTI and ETI are initiated in different host cell compartments, they rely on the transcriptional activation of similar sets of genes, suggesting pathway convergence upstream of nuclear events. We report that PTI triggered by Arabidopsis LRR-RP (RLP23) requires signalling-competent dimers of the lipase-like proteins EDS1 and PAD4, and ADR1-family helper NLRs, which are all components of ETI. The cell surface LRR-RK SOBIR1 links RLP23 with EDS1, PAD4 and ADR1 proteins, suggesting formation of constitutive supramolecular complexes containing PTI receptors and transducers at the inner side of the plasma membrane.

2021-07-05 | PXD026120 | Pride

Isolation of Arabidopsis EDS1 complexes from leaves with activated TNL RPS4/RRS1 signaling

Project description:Nod-like receptors (NLRs) belong to AAA+ ATPases and act as ATP-dependent molecular switches, which detect activity of pathogens. Function of a large class of plant NLRs with Toll-like domain (TNL) is fully dependent on a class of EDS1-like proteins specific to seed plants. EDS1 (Enhanced Disease Susceptibility 1) – like proteins are defined as fusions of two domains: lipase-like a/b hydrolase domain and an a-helical bundle domain specific to the EDS1 family. In Arabidopsis, RRS1-RPS4 TNL signaling is dependent on the nuclear localization of EDS1 and formation of heterodimers between EDS1 and its sequence-related partners, PAD4 and SAG101. Here, we deposited results of affinity purification and LC-MS analyses are deposited for the EDS1 complexes after triggering NLR-dependent immune responses in Arabidopsis leaves. As a negative control, plants expressing GFP-tagged Telomere Repeat Binding 1 were used. The complexes were purified from nuclear enriched fractions of Arabidopsis complementation lines infected with the TNL-triggering bacteria.

2021-04-06 | PXD025202 | Pride

Cavity surface residues of PAD4 and SAG101 contribute to EDS1 dimer signaling specificity in plant immunity

Project description:Arabidopsis pathogen effector-triggered immunity (ETI) is controlled by a family of three lipase-like proteins EDS1, PAD4 and SAG101 and two sub-families of HET-S/LOB-B (HeLo)-domain “helper” NLRs, ADR1s and NRG1s. EDS1-PAD4 dimers cooperate with ADR1s, and EDS1-SAG101 dimers with NRG1s, in two separate defense-promoting modules. EDS1-PAD4-ADR1 and EDS1- SAG101-NRG1 complexes were detected in immune-activated leaf extracts but the molecular determinants for specific complex formation and function remain unknown. EDS1 signaling is mediated by a C-terminal EP domain (EPD) surface surrounding a cavity formed by the heterodimer. Here we investigated whether the EPDs of PAD4 and SAG101 contribute to EDS1 dimer functions. Using a structure-guided approach, we undertook a comprehensive mutational analysis of Arabidopsis PAD4. We identify two conserved residues (Arg314 and Lys380) lining the PAD4 EPD cavity that are essential for EDS1-PAD4 mediated pathogen resistance, but are dispensible for PAD4 mediated restriction of green peach aphid infestation. Positionally equivalent Met304 and Arg373 at the SAG101 EPD cavity are required for EDS1-SAG101 promotion of ETI-related cell death. In a PAD4 and SAG101 interactome analysis of ETI-activated tissues, PAD4R314A and SAG101M304R EPD variants maintain interaction with EDS1 but lose association, respectively, with helper NLRs ADR1-L1 and NRG1.1, and other immune-related proteins. Our data reveal a fundamental contribution of similar but non-identical PAD4 and SAG101 EPD surfaces to specific EDS1 dimer protein interactions and pathogen immunity.

2022-03-17 | PXD029477 | Pride

Transcription profiling of Arabidopsis leading to the identification of novel components in the EDS1/PAD4-regulated defence pathwayabidopsis-Pst-eds1-pad4

Project description:Gene expression profiling leading to the identification of novel components in the EDS1/PAD4-regulated defence pathway

2006-02-15 | E-MEXP-546 | biostudies-arrayexpress