Transcriptomics

Dataset Information

20

Wnt & LIF signalling in mESC


ABSTRACT: The molecular processes underlying the properties of ESC are yet unknown even when it's well established that LIF/STAT3 is neccesary for the maintenance of pluripotency. Other pathways as Wnt are may be implicated in the regulation of the biological mechanisms in mESC. Work model: D3-ES cultivated with or without LIF and treated with chronic (7 days) low doses (50nM) of GSK3 β inhibitor (lithium). We used microarrays to detail the global profile of gene expression to identified different groups of up and down-regulated genes in the work model. Overall design: mESC cultivated as it has been defined in the work model were prepared for RNA extraction and hybridization on Affymetrix microarrays. We analyzed 3 independent experiments per replicate, and we did three replicates per sample.

INSTRUMENT(S): [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array

ORGANISM(S): Mus musculus  

SUBMITTER: Patricia Ruiz-Ontañón  

PROVIDER: GSE11628 | GEO |

SECONDARY ACCESSION(S): PRJNA106143

REPOSITORIES: GEO

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