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Interaction of soil amoeba Protostelium aurantium with three pathogenic yeasts Candida spp. (here: C. albicans and C. glabrata)

ABSTRACT: We investigated different escape and defense strategies of Candida glabrata and Candida albicans against the natural fungivorous predator Protostelium aurantium using dual RNA-Seq. While C. albicans was found to be very succesfull in preventing from amoeba recognition, C. glabrata in turn, was rapidly taken up but remained undigested with a comparably low transcriptional activity. Trophozoites of the amoeba Protostelium aurantium were fed seperately with the two fungal species and samples for RNA isolation were taken at time points 0 min (control), 30 min and 60 min. At indicated time points samples were shock frozen and used for RNA isolation. Samples for RNAseq were taken at time points 0 min (control), 30 min and 60 min. RNA samples from identical timepoints for C. albicans and C. glabrata were pooled for RNA sequencing. We investigated different escape and defense strategies of Candida spp. against natural fungivorous predator. While C. parapsilosis underwent rapid predation and intracellular killing of the yeast, C. albicans was found to be very succesfull in preventing from amoeba recognition. C. glabrata in turn, was rapidly taken up but remained undigested with a comparably low transcriptional activity.

ORGANISM(S): Candida albicans Planoprotostelium fungivorum Nakaseomyces glabratus

PROVIDER: GSE116533 | GEO | 2018/09/01

REPOSITORIES: GEO

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Interaction of soil amoeba Protostelium aurantium with three pathogenic yeasts Candida spp. (here: C. parapsilosis)

Project description:We investigated different escape and defense strategies of Candida parapsilosis against the natural fungivorous predator Protostelium aurantium using dual RNA-Seq. Trophozoites of the amoeba Protostelium aurantium were fed with C. parapsilosis and samples for RNA isolation were taken at time points 0 min (control), 30 min and 60 min. At indicated time points samples were shock frozen and used for RNA isolation. C. parapsilosis underwent rapid predation and intracellular killing of the yeast

2018-09-01 | GSE116534 | GEO

[URE3] Prion formation by the Candida albicans Ure2p (but not by C. glabrata Ure2p)

Project description:This SuperSeries is composed of the following subset Series: GSE26611: [URE3] Prion formation by the Candida albicans Ure2p (but not by C. glabrata Ure2p)-ScUre2 GSE26612: [URE3] Prion formation by the Candida albicans Ure2p (but not by C. glabrata Ure2p)-CgUre2 GSE26613: [URE3] Prion formation by the Candida albicans Ure2p (but not by C. glabrata Ure2p)-CaUre2 Refer to individual Series

2012-01-01 | E-GEOD-26620 | biostudies-arrayexpress

Homo sapiens whole blood infected with Candida spp. (here: Candida glabrata)

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2018-12-26 | GSE114175 | GEO

Milbemycins: more than efflux inhibitors for fungal pathogens

Project description:This study investigated the effect of milbemycons as efflux inhibitors and antifungal agents. Milbemycin oxims can inhibit growth of Candida glabrata and C. albicans. The effect of milbemycins on transcriptomes was inbvestigated.

2013-03-08 | GSE40232 | GEO

Identification and mode of action of a plant natural product targeting human fungal pathogens

Project description:Candida albicans was subjected to treatment with tomatidine and samples were taken at 2 different time points (1h, 3h). The results show that tomatidine induces genes involved in ergosterol biosynthesis as compared to untreated controls.

2017-06-28 | GSE96965 | GEO

Limosilactobacillus fermentum Limits Candida glabrata Growth by Ergosterol Depletion

Project description:Candida glabrata is a human-associated opportunistic fungal pathogen. It shares its niche with Lactobacillus spp. in the gastrointestinal and vaginal tract. In fact, Lactobacillus species are thought to competitively prevent Candida overgrowth. We investigated the molecular aspects of this antifungal effect by analyzing the interaction of C. glabrata strains with Limosilactobacillus fermentum. From a collection of clinical C. glabrata isolates, we identified strains with different sensitivities to L. fermentum in coculture. We analyzed the variation of their expression pattern to isolate the specific response to L. fermentum. C. glabrata-L. fermentum coculture induced genes associated with ergosterol biosynthesis, weak acid stress, and drug/chemical stress. L. fermentum coculture depleted C. glabrata ergosterol. The reduction of ergosterol was dependent on the Lactobacillus species, even in coculture with different Candida species. We found a similar ergosterol-depleting effect with other lactobacillus strains (Lactobacillus crispatus and Lactobacillus rhamosus) on Candida albicans, Candida tropicalis, and Candida krusei. The addition of ergosterol improved C. glabrata growth in the coculture. Blocking ergosterol synthesis with fluconazole increased the susceptibility against L. fermentum, which was again mitigated by the addition of ergosterol. In accordance, a C. glabrata Derg11 mutant, defective in ergosterol biosynthesis, was highly sensitive to L. fermentum. In conclusion, our analysis indicates an unexpected direct function of ergosterol for C. glabrata proliferation in coculture with L. fermentum.

2023-02-08 | GSE202656 | GEO

Label-free quantitative proteomics in Candida yeast species: technical and biological replicates to asses data reproducibility

Project description:Objective Label-free quantitative proteomics has emerged as a powerful strategy to obtain high quality quantitative measures of the proteome with only a very small quantity of total protein extract. Because our research projects were requiring the application of bottom-up shotgun Mass Spectrometry proteomics in the pathogenic yeasts Candida glabrata and Candida albicans, we performed preliminary experiments to i) obtained a precise list of all the proteins for which measures of abundance could be obtain and ii) assess the reproducibility of the results arising respectively from biological and technical replicates. Data description C. glabrata and C. albicans cells were grown in minimal liquid medium. Three independent time-courses were performed simultaneously, in each species, and an alkaline pH stress was induced for two of them. Cells were collected 10 and 60 minutes after stress induction and proteins were extracted. They were analysed and quantified two time by mass spectrometry. Our final dataset thus comprises label-free quantitative proteomics results for 24 samples (two species, three time-courses, two time points and two runs of mass spectrometry). Statistical procedures were applied to identify proteins with differential abundances between stressed and unstressed situations. Considering that C. glabrata and C. albicans are human pathogens which face important pH fluctuations during a human host infection, this dataset has a potential value to other researchers in the field.

2019-07-08 | PXD014125 | Pride

Divergent mitochondrial responses and metabolic signal pathways secure the azole resistance in Crabtree-positive and negative Candida species

Project description:we performed mitochondrial proteomic analysis on multiple Candida species (C. albicans, C. glabrata, C. krusei and Candida auris) and analyzed the differentially expressed mitochondrial proteins (DEMPs) between azole-sensitive and azole-resistant Candida species.

2023-09-05 | PXD045101 | iProX

Expression profiling of C. albicans and C. dubliniensis in reconstituted human epithelium

Project description:This SuperSeries is composed of the following subset Series: GSE13318: Expression profiling of Candida albicans and Candida dubliniensis in reconstituted human oral epithelium 30 min p.i. GSE13345: Expression profiling of Candida albicans and Candida dubliniensis in reconstituted human oral epithelium 90 min p.i. GSE13352: Comparative expression profiling in Candida albicans and Candida dubliniensis in inocula, RHE, and PCF cultures Refer to individual Series

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Effect of fludioxonil treatment on candida albicans SC5314

Project description:Comparison of Candida albicans SC5314 treated with fludioxonil for 30 min and untreated controls under hyphae-inducing conditions

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