Dataset Information


Oct3/4 expression and notch signaling in bone marrow stromal cells reprogrammed by Xenopus egg extract

ABSTRACT: Bone marrow stromal cells (BMSCs) are multipotent stem cells that preferentially differentiate into mesenchymal cells. If they can be dedifferentiated into embryonic stem cell-like cells, they will be a highly attractive source for cell therapy. Cell and egg extracts have been used in a few studies to evaluate nuclear reprogramming, but these have not examined cell pluripotency in any detail. In this study, we used a cell reversible permeabilization method to treat BMSC with Xenopus laevis mitotic egg extract. We observed an upregulation of the pluripotent protein Oct3/4 in BMSCs treated by this extract. We also further evaluated transcriptional changes with a focused stem cell oligonucleotide array. A number of genes involved in the Notch or Wnt signaling pathways were upregulated in BMSC exposed to Xenopus egg extract. In conclusion, our microarray data from BMSCs exposure to egg extracts may provide interesting clues regarding factors involved in nuclear reprogramming. Our approach is an alternative method towards dedifferentiation of cells without genetic modification, which is preferable in the clinical situation. Keywords: Cell type comparison Overall design: Expression profiling was performed on murine bonemarrow stromal cells cultured in-vitro versus stromal cells exposed to Xenopus extract and compared with murine embryonic stem cells

INSTRUMENT(S): SuperArray Mouse stem cell oligonucleotide array

ORGANISM(S): Mus musculus  

SUBMITTER: Vanessa Hall 

PROVIDER: GSE11660 | GEO | 2008-07-11



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