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The analysis of gene expression profiles of splenocytes from wild type and UBL4A/GdX-/Y mice upon LPS stimulation

ABSTRACT: Purpose: To compared the gene expression profiles of splenocytes from wild type and UBLA4/GdX (Ubiquitin-like protein 4A, also named GdX) knockout mice upon LPS stimulation. Methods: GdX+/Y and GdX-/Y mice were injected with LPS. Their splenocytes were collected at different time (90min or 6 hrs), and then performed RNA-sequencing analysis. We sequence the samples used BGISEQ-500 platform. Results: The average mapping ratio with reference genome is 93.42%, the average mapping ratio with gene is 83.40%; A total of 17,920 genes were detected.A number of NF-κB targeting genes, such as IL-6, IL-1a and IL-12, have dramatic higher expressions in the splenocytes of GdX+/Y mice than that of GdX-/Y mice. Gene ontology (GO) analysis of GdX function in LPS-treated mice showed one of the most significantly enriched biological processes is related to inflammatory response. Conclusions: Our study revealed the gene expression profiles of the splenocytes from LPS-treated wild type and GdX-/Y mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE116956 | GEO | 2019/03/20

REPOSITORIES: GEO

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