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SMRT-Cappable-seq reveals complex operon variants in bacteria

ABSTRACT: We develop a method SMRT-Cappable-seq that combines the isolation of unfragmented bacterial primary transcripts with the longread sequencing using PacBio. This method allows the identification and phasing of the transcrription start sites and the termination sites, thereby revealing the operon structure and the regulation of gene experession in bacteria. Applied to E.coli, our method results in an unprecedented definition of the transcriptome with 34% of the known operons from RegulonDB database being extended by at least one gene, and identifies a total of 2300 operons from which around 900 are novel.

ORGANISM(S): Escherichia coli

PROVIDER: GSE117273 | GEO | 2018/07/22

REPOSITORIES: GEO

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