Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of the genotoxic human lung carcinogen benzo[a]pyrene and its non-carcinogenic isomer benzo[e]pyrene on transcriptomic profiles in HepaRG cells.

ABSTRACT: Twenty-eight days after the initial seeding, the terminally differentiated HepaRG cells were treated with 2 µM benzo[a]pyrene (B[a]P) and benzo[e]pyrene (B[e]P) for 72 hours. Following the treatment, the cells were harvested by mild trypsinization, washed in phosphate-buffered saline, and immediately frozen at −80°C for subsequent analyses. Gene expression profiles in the HepaRG cells treated with B[a]P and B[e]P were investigated using Agilent whole genome 8x60K human microarrays according to the manufacturer’s instructions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE117476 | GEO | 2018/07/24

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Gene expression profiling after a chronic exposure to benzo[a]pyrene (B[a]P) and/or ethanol in steatotic or non-steatotic HepaRG cells.

Project description:The aim of this study was to determine in the human HepaRG cell line whether lipid overload (steatosis) could favor the cytotoxicity of benzo[a]pyrene (B[a]P) alone or in association with ethanol.

2018-04-18 | GSE102536 | GEO

Effect of the genotoxic human lung carcinogen benzo[a]pyrene and its non-carcinogenic isomer benzo[e]pyrene on transcriptomic profiles in HepaRG cells.

Project description:Effect of the genotoxic human lung carcinogen benzo[a]pyrene and its non-carcinogenic isomer benzo[e]pyrene on transcriptomic profiles in HepaRG cells.

| PRJNA482305 | ENA

Cytochrome P450 Monooxygenase Mediated Metabolic Utilization of Benzo(a)pyrene by Fungi

Project description:Soil dwelling Aspergillus fungi possess the versatile metabolic capability to utilize complex organic compounds which are toxic to humans, yet the mechanisms they employ remain largely unknown. Benzo(a)pyrene is a common carcinogenic contaminant, posing a significant concern for human health. Here, we report that Aspergillus fungi can degrade benzo(a)pyrene effectively. In Aspergillus nidulans, exposure to benzo(a)pyrene results in transcriptomic and metabolic changes associated with cellular growth and energy generation, implying that the fungus utilizes benzo(a)pyrene as a food. Importantly, we identify and characterize the conserved bapA gene encoding a cytochrome P450 monooxygenase that exerts the first step in the degradation of benzo(a)pyrene. We further demonstrate that the fungal NF-κB-type global regulators VeA and VelB are required for benzo(a)pyrene degradation in A. nidulans, which occurs through expression control of bapA in response to nutrient limitation. Our study illuminates fundamental knowledge of fungal benzo(a)pyrene metabolism and provides novel insights into enhancing bioremediation potential.

2018-07-10 | GSE116804 | GEO

Glutathione S-transferase induction after exposure to benzo[a]pyrene

Project description:Benzo[a]pyrene induction of glutathione S-transferases, an activity-based protein profiling investigation

2020-05-26 | PXD010610 | Pride

Acute hepatic transcriptional response in male mice following oral exposure to benzo(a)pyrene

Project description:Benzo(a)pyrene is a well-established human carcinogen in humans and rodents. In the present study, we sought to determine the dose- and time-dependent changes in gene expression upon oral exposure to benzo(a)pyrene. Adult male B6C3F1 mice were exposed to four doses of benzo(a)pyrene or vehicle control for three days and sacrificed 4 or 24 hours after the final exposure.

2015-07-01 | GSE43633 | GEO

Modulating the Response to Benzo[a]pyrene when CYP1A1 and CYP1B1 are Knocked Down

Project description:Benzo[a]pyrene is a Group 1 carcinogen. It undergoes metabolism in the liver through CYP1A1 and CYP1B1 enzymes. This study was conducted to investigate the response to benzo[a]pyrene in the liver when these enzymes are knocked down.

2024-01-03 | GSE252339 | GEO

Sub-chronic forestomach transcriptional response in adult male MutaTMMouse following oral exposure to benzo(a)pyrene

Project description:Benzo(a)pyrene is a well-established human carcinogen in humans and rodents. In the present study, we sought to determine the dose- and time-dependent changes in gene expression upon oral exposure to benzo(a)pyrene. Adult male MutaTMMouse were exposed to three doses of benzo(a)pyrene or vehicle control (olive oil) for 28 days and sacrificed three days after the final exposure.

2013-09-27 | GSE43438 | GEO

Transcriptomic response to benzo[a]pyrene treatment in HepG2 cells

Project description:This SuperSeries is composed of the following subset Series: GSE36242: Transcriptomic response to benzo[a]pyrene treatment in HepG2 cells (RNA-Seq) GSE36243: Transcriptomic response to benzo[a]pyrene treatment in HepG2 cells (Affymetrix) Refer to individual Series

2012-11-16 | E-GEOD-36244 | biostudies-arrayexpress

Tempo-Seq targeted RNA sequencing of the HepaRG cell line treated with aflatoxin B1, benzo[a]pyrene, cyclosporin A, rotenone and thrichostatin A over a range of chemical concentrations and exposure time points

Project description:The experiment investigates the effects of five well-known chemicals on the transcriptome of the HepaRG cell line, a metabolically competent hepatic cell line. The cells were treated individually with an increasing concentrations of aflatoxin B1, benzo[a]pyrene, cyclosporine A, rotenone or trichostatin A at five exposure time points followed by targeted RNA-seq using TempO-Seq technology (the panel of human whole transcriptome). The aim of the study was to explore how and to what extent the point-of-departure (POD) obtained from an in vitro transcriptomics study varied as a function of exposure time.

2023-08-01 | E-MTAB-13168 | biostudies-arrayexpress

Benzo[a]pyrene response in primary mouse hepatocytes

Project description:Gene expression changes to several concentrations of benzo[a]pyrene in primary hepatocytes of wildtype and Xpa-/-p53+/- mice

2011-08-11 | E-TABM-1139 | biostudies-arrayexpress