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MicroRNAs are negative and positive post-transcriptional regulators of BRAF-X1 mRNA

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE117642 | GEO | 2019/03/31

REPOSITORIES: GEO

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microRNAs are negative and positive post-transcriptional regulators of BRAF-X1 mRNA [miRNA]

Project description:X1 is the most abundant transcript variant of BRAF mRNA and has a long 3’UTR, but its involvement in post-transcriptional regulatory circuits has not been investigated yet. Here, we describe 20 microRNAs that bind directly to the X1 3’UTR. They were identified in BRAFV600E mutant A375 melanoma cells using miR-CATCHv2.0, an implemented experimental method that combines RNA affinity purification with small RNA sequencing and an ad hoc analytical workflow. X1-targeting microRNAs fall into 4 classes, according to the effect that they exert (decrease/increase in BRAFV600E mRNA and protein levels) and on the mechanism they use to achieve it (destabilization/stabilization of X1 mRNA or decrease/increase in its translation). In many cases, the microRNA-induced variations in BRAFV600E protein levels are coupled to consistent variations in pMEK levels and, in turn, to melanoma cell proliferation and sensitivity to the BRAF inhibitor vemurafenib. However, examples exist of microRNAs that uncouple the degree of activation of the ERK pathway from the levels of BRAFV600E protein. Our study describes miR-CATCHv2.0 as an effective tool for the identification of direct microRNA-target interactions and unveils the complexity of the post-transcriptional regulation to which BRAFV600E and the ERK pathway are subjected in melanoma cells.

2019-03-31 | GSE117641 | GEO

microRNAs are negative and positive post-transcriptional regulators of BRAF-X1 mRNA [miR-CATCH]

2019-03-31 | GSE117640 | GEO

microRNAs are negative and positive post-transcriptional regulators of BRAF-X1 mRNA

Project description:microRNAs are negative and positive post-transcriptional regulators of BRAF-X1 mRNA

| PRJNA482818 | ENA

microRNAs are negative and positive post-transcriptional regulators of BRAF-X1 mRNA [miRNA]

Project description:microRNAs are negative and positive post-transcriptional regulators of BRAF-X1 mRNA [miRNA]

| PRJNA482819 | ENA

Systematic evaluation of the microRNAome through miR-CATCHv2.0 identifies positive and negative regulators of BRAF-X1 mRNA.

Project description: Not available

| S-EPMC6546394 | biostudies-literature

microRNAs are negative and positive post-transcriptional regulators of BRAF-X1 mRNA [miR-CATCH]

Project description:microRNAs are negative and positive post-transcriptional regulators of BRAF-X1 mRNA [miR-CATCH]

| PRJNA482822 | ENA

Transcriptional Regulators Compete with Nucleosomes Post-replication.

Project description: Not available

| S-EPMC4855302 | biostudies-literature

Cardiac ReductomiRs: Identification of Novel Nrf2-Dependent microRNAs as Post-Transcriptional Regulators of Myocardial Gene Expression

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-04-01 | GSE120089 | GEO

Response of BRAF-mutant melanoma to BRAF inhibition is mediated by a network of transcriptional regulators of glycolysis.

Project description: Not available

| S-EPMC4110245 | biostudies-literature

Cardiac ReductomiRs: Identification of Novel Nrf2-Dependent microRNAs as Post-Transcriptional Regulators of Myocardial Gene Expression [mRNA]

Project description:Background: Although modern advances in the treatment of heart disease have reduced mortality rates, long-term medical management is often associated with a progressive loss of function that manifests as heart failure. Nuclear factor, erythroid 2 like 2 (Nfe2l2, a.k.a. Nrf2) is a transcriptional regulator that provides the ischemic heart with transient cytoprotection following acute myocardial insult; however, its sustained activation paradoxically causes reductive stress, an emerging pathological phenomenon characterized by excessive antioxidant function and proteotoxic remodeling. Yet, the molecular mechanism by which chronic reductive stress produces heart failure remains elusive. Methods: Cardiac-specific constitutively active Nrf2 transgenic (caNrf2-Tg) mice expressing the transgene at low (TgL) and high (TgH) levels were employed in integrative mRNA and small RNA (miRNA) sequencing. Results and Conclusion: Here, we reveal several Nrf2 dose-responsive miRNA loci harboring putative upstream antioxidant response elements (AREs). Intriguingly, Nrf2-mediated miRNA responses were distinctly enriched for post-transcriptional regulation of cardiac-specific mRNAs. Altogether, these data reveal Nrf2 as a potent regulator of cardiac miRNA expression and provide novel candidates for future mechanistic investigation into the relationship between myocardial redox status and pathophysiology.

2020-04-01 | GSE120087 | GEO

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