Identification of microRNA-668 and microRNA-489 targets by RISC immunoprecipitation

ABSTRACT: MicroRNAs inhibits their target mRNA translation by forming RISC complex. Immunoprecipitation of RISC will pull down both microRNA and their target mRNAs. To systemetically examine the potential targets of microRNA-668 and microRNA-489, HEK293 cells were overexpressed with negative control, microRNA-668, or microRNA-489 mimics. Both total RNA samples and RNA samples from RISC complex pulled down by Ago-2 immunoprecipitation were examined by RNA-seq.

ORGANISM(S): Homo sapiens

PROVIDER: GSE117805 | GEO | 2019/07/27

REPOSITORIES: GEO