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Global gene expression analysis of primary head and neck cancer cases

ABSTRACT: This study addressed the assumption that in addition to somatic mutations epigenetic silencing of cancer-related genes by promoter hyper-methylation is a common event in head and neck cancer. A multi-scale omics approach based on whole-exome sequencing, global DNA methylation and gene expression profiling data derived from tumor samples of the HIPO-HNC cohort was conducted and new findings were confirmed with data from TCGA-HNC. Results provide experimental evidence that the ryanodine receptor 2 (RYR2) exhibits variable promoter methylation and expression in both tumor samples and HNSCC cell lines. Moreover, immunohistochemical staining of tissue sections unraveled a gradual loss of RYR2 expression from normal mucosa via dysplastic lesions to cancer, and indicated that reduced RYR2 expression in adjacent tissue and precancer lesions might serve as a risk factor for unfavorable prognosis and upcoming malignant conversion.

ORGANISM(S): Homo sapiens

PROVIDER: GSE117973 | GEO | 2020/06/19

REPOSITORIES: GEO

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Identification of epigenetically silenced RYR2 by DNA methylation profiling in head and neck cancer squamous cell carcinoma

Project description:RYR2 promoter methylation and its impact on RYR2 gene expression was investigated in a cohort of patients with head and neck squamous cell carcinoma. The results suggest that impaired RYR2 function is a common event in HNSCC pathogenesis.

2021-06-11 | E-MTAB-7468 | biostudies-arrayexpress

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2023-08-30 | PXD042295 | Pride

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2022-01-21 | GSE194073 | GEO

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Phosphorylation-dependent Interactome of Ryanodine Recep-tor Type-2 in the Heart

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2021-06-07 | PXD026350 | Pride

RyR2/IRBIT regulates insulin gene transcription, insulin content, and secretion in the insulinoma cell line INS-1

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