Characterizing the gene expression profile of Prox1+ intestinal adenoma organoid cells

ABSTRACT: We isolated and selected intestinal adenoma organoids from Apcmin/+; Rosa26LSL-TdTomato; Prox1-CreERT2 mice. After the selection procedure without growth factors, we induced CreERT2 activity and the transcription of tdTomato to label Prox1+ cells by 300 nM 4-hydroxytamoxifen for 16h. tdTomato+ (Prox1+) and tdTomato- cells (enriched for Prox1- cells) were FACS sorted and total RNA was isolated.

ORGANISM(S): Mus musculus

PROVIDER: GSE117981 | GEO | 2018/08/21

REPOSITORIES: GEO