Single cell changes in DBZ or DMSO treated cells derived from Apc-mutant organoid cultures

ABSTRACT: We made intestinal organoid cultures from Villin-CreERT2;Apcflox/flox mice. Organoids were subjected to 4-OH-Tam treatment, whereafter Apc mutant organoids were selected in R-Spondin free medium. DBZ or DMSO treatment was started on day 3. On day 8, organoids were dissociated to obtain single cells and analyzed using the Chromium Single-cell 3'RNA-sequencing system

ORGANISM(S): Mus musculus

PROVIDER: GSE118055 | GEO | 2018/08/21

REPOSITORIES: GEO