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Expression data of ex vivo purified CD4+ cells from WT and P2rx7-/- mice

ABSTRACT: Extracellular adenosine triphosphate (eATP) is a signaling molecule that affects T cell function via the ionotropic P2X7 receptor. The study of effector/memory T cells isolated from mice with deletion of P2rx7, the gene encoding for P2X7, allowed understanding the impact of P2X7 activity on T cell function in the eATP-rich tumor microenvironment. To explore the the transcriptional impact of the lack of P2rx7 in CD4+ naïve and TEM cells, we performed genome-wide expression profiling of ex vivo purified CD4+ naïve and TEM cells from WT and P2rx7-/- mice

ORGANISM(S): Mus musculus

PROVIDER: GSE118146 | GEO | 2020/07/24

REPOSITORIES: GEO

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T cell-specific P2RX7 favors lung parenchymal CD4+ T cell accumulation in response to severe lung infections

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P2X7 signaling drives Th1 cell differentiation through metabolic reprogramming for aerobic glycolysis

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Gene expression profile at single cell level of tumor-infiltrating immune cells from mice treated with anti-PD-L1+E.colipBAD28 vs anti-PD-L1+E.colipApyr [CD45positive_TILs_scRNAseq]

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RNA-seq comparison of whole muscle between WT mice, dystrophic mice, and P2rx7-null dystrophic mice

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2019-09-26 | E-MTAB-7698 | biostudies-arrayexpress

Genetic deletion of P2X7 receptors causes genome-wide alteration of gene expression and results in antidepressant phenotype in mice amygdale

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2011-03-31 | E-GEOD-21218 | biostudies-arrayexpress

Extracellular ATP elicits DORN1-mediated RBOHD phosphorylation to regulate stomatal aperture

Project description:A multibillion dollar pharmaceutical industry targets purinergic signaling pathways in humans. However, in contrast, little is known about purinergic signaling in plants. Extracellular adenosine-5’-triphosphate (eATP) acts as a damage-associated molecular pattern (DAMP) in both animals and plants. The first plant eATP receptor, DORN1, was identified in Arabidopsis, defining a new kinase family (P2K) of purinoceptors. Stomates are leaf pores that control gas exchange and, therefore, impact critical functions such as photosynthesis and drought tolerance. Stomates are also the prefer entry point for leaf pathogens. The addition of ATP led to the rapid closure of Arabidopsis leaf stomates resulting in enhanced resistance to the bacterial pathogen Psuedomonas syringae. The data indicate that this response is mediated by eATP recognition by DORN1 followed by direct phosphorylation of the NADPH oxidase RBOHD, resulting in elevated production of reactive oxygen species and stomatal closure. Mutation of the DORN1 phosphorylation sites on RBOHD eliminated the ability of eATP to induce stomatal closure. To date, in contrast to work in animals, purinergic signaling in plants has been viewed as a curiosity without any direct, mechanistic connection to key plant processes. The data presented clearly demonstrates eATP as an important signal controlling stomatal aperture with important implications for the control of plant photosynthesis, water homeostasis, pathogen resistance and ultimately yield.

2018-10-24 | PXD006678 | Pride

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