Transcriptomics

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Single cell dissection of Chemical Reprogramming from Fibroblasts into functional Chondrocytes


ABSTRACT: We used single cell analysis to systematically dissect the sequential changes of cellular phenotypes during fibroblast-chondrocyte direct reprograming.1401 single-cell transcriptomes were measured in total (232 MEFs of day0, 577 chemical-induced intermediate cells (ci-ICs) of day6, 311 ci-chons of day 34, 281 mouse primary chondrocytes (mchons) as positive control). We found that ci-chons and mchons shared similar subpopulation with specifically expression of cartilage-related markers. The findings also indicated that in the early induction, fibroblasts to lose their original features, and transit into a cartilage progenitor intermediate state.

ORGANISM(S): Mus musculus

PROVIDER: GSE118234 | GEO | 2018/11/19

REPOSITORIES: GEO

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