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Short trichome comparative genomic hybridization analysis in glycine max fast neutron mutants

ABSTRACT: One short trichome mutant (family known by multiple names, including R59C46, FN0175946, and FNMN0409) was identified in a soybean fast neutron population (known as VP02 in Bolon et al. 2011). An advanced line of this mutant was compared to its wild-type parent (M92-220-Long) using CGH to identify the causative region/gene associated with the short trichome phenotype.

ORGANISM(S): Glycine max

PROVIDER: GSE118251 | GEO | 2018/08/08

REPOSITORIES: GEO

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Comparative genomic hybridization analysis in glycine max fast neutron mutants

Project description:Soybean fast neutron mutant lines were maintained to an advanced generation (ranging between approximately M6 and M11) and compared to their wild-type parent (M92-220-Long) using CGH to identify sequence deletions and duplications in the mutant plants.

2018-08-16 | GSE118590 | GEO

Seed composition traits: comparative genomic hybridization analysis in glycine max fast neutron mutants

Project description:Ten mutants with altered seed composition traits were identified in a soybean fast neutron population (Bolon et al. 2014). These mutant lines were maintained to an advanced generation (ranging between M5 and M9) and compared to their wild-type parent (M92-220-Long) using CGH to identify the causative region/gene associated with the seed composition changes.

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High-sucrose/low-oil comparative genomic hybridization analysis in glycine max fast neutron mutants

Project description:Two high-sucrose/low-oil mutants (FN0176450/2012CM7F040P06/SRX826343 and FN0176450/2012CM7F040P05/SRX826351) were identified in a soybean fast neutron population (Bolon et al. 2014). These mutant lines were then advanced to the M3:7 generation and compared to thier wild-type parent (M92-220-Long) using CGH to identify the causative region/gene associated with high-sucrose/low-oil.

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Soybean fast neutron genotyping: 700k Forward genetics

Project description:CGH was used to compare induced structural variation among soybean fast neutron lines, developed in background M92-220. 114 different soybean fast neutron lines were hybridized against the parental reference genotype M92-220. The soybean tiling array consists of 700k probes, spaced at approximately 1.1 kb intervals.

2011-08-01 | E-GEOD-31038 | biostudies-arrayexpress

Soybean fast neutron genotyping: 700k Reverse genetics

Project description:CGH was used to compare induced structural variation among soybean fast neutron lines, developed in background M92-220. 11 different soybean fast neutron lines were hybridized against the parental reference genotype M92-220. The soybean tiling array consists of 700k probes, spaced at approximately 1.1 kb intervals.

2011-08-02 | E-GEOD-31111 | biostudies-arrayexpress

Soybean fast neutron genotyping: 700k Reverse genetics

Project description:CGH was used to compare induced structural variation among soybean fast neutron lines, developed in background M92-220.

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Short trichome comparative genomic hybridization analysis in glycine max fast neutron mutants

Project description:Short trichome comparative genomic hybridization analysis in glycine max fast neutron mutants

| PRJNA484957 | ENA

CGH: FN1.4_v_M92-220

Project description:Soybean fast neutron (FN) mutant lines were compared by CGH to catalog structurally variant (e.g. deletion and duplication) regions compared to the parent line (M92-220). The CGH comparisons reveal reveal putative deletions and duplications among the mutant lines. FN lines were compared through a common reference, M92-220 (this is a sub-line individual isolated from the M92-220 seed stock). Each FN line was represented by a single individual plant. The reported values show the log2 ratio of the normalized FN line hybridization signals (Cy3) over the normalized M92-220 hybridization signal (Cy5).

2014-06-04 | E-GEOD-58172 | biostudies-arrayexpress

CGH: FN1.4_v_M92-220

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