Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Sequencing of microglia during focal remyelination of the corpus callosum

ABSTRACT: The goal of the study was to determine the gene expression of microglia prior to the onset of remyelination (3 days post lysolecithin injection) and pro-regenerative microglia at the onset of remyelination (10 days post remyelination).

ORGANISM(S): Mus musculus

PROVIDER: GSE118450 | GEO | 2019/05/24

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Sequencing of microglia during focal remyelination of the corpus callosum

Project description:Sequencing of microglia during focal remyelination of the corpus callosum

| PRJNA485693 | ENA

MicroRNA profiling of demyelination and remyelination areas in corpus callosum of cuprizone-treated mice

Project description:Mouse cuprizone (CPZ ) model of experimental de- and remyelination was applied to mimic demyelination pathology of multiple sclerosis. In order to identify differentially expressed microRNAs involved in de- and remyelination, the affected areas of corpus callosum were isolated from mice exposed to CPZ and conducted an Agilent microarray analysis. To induce demyelination, CPZ was administrated for four weeks. Spontaneous remyelination occurs as mice returned to the regular diet after four weeks feeding with CPZ (DEM_4w). Remyelination was examined at two time points: acute remyelination induced by four weeks CPZ feeding followed by two days of regular diet (two days remyelination: REM_2d), and full remyelination induced by four weeks CPZ feeding followed by two weeks of regular diet (two weeks remyelination: REM_2w). Control mice (C) were kept on a normal diet. The following groups representing de- and remyelinisation pathology in corpus callosum of CPZ-treated mice were compared: Demyelination: 4weeks CPZ: DEM_4w; Acute remyelination: 4 weeks CPZ +2 days UNTREATED: REM_2d; Full remyelination: 4 weeks CPZ +2 weeks UNTREATED: REM_2w; and UNTREATED control (C). The experiments were performed using 2-4 animals per groups.

2018-04-11 | GSE100662 | GEO

Gene expression after focal demyelination of the corpus callosum

2010-04-13 | GSE21310 | GEO

TRAP-seq of mouse astrocytes involved in central nervous system remyelination

Project description:Remyelination failure contributes to axonal dysfunction in neurodegenerative disorders. But whether astrocytes, the most abundant glial cell type in demyelinated lesions, support or impede remyelination is controversial. Following focal demyelinated lesions of the mouse corpus callosum induced with the myelin toxin lysolecithin, we used TRAP (translational ribosome affinity purification) sequencing to isolate and sequence ribosome-associated mRNAs which are being actively translated in astrocytes, and studied how the responses and molecular mechanisms in astrocytes are linked to remyelination.

2023-05-17 | E-MTAB-9270 | biostudies-arrayexpress

Gene expression after focal demyelination of the corpus callosum

Project description:To determine expression profiles of cytokines and growth factor profiles in the subventricular zone (SVZ) after demyelination we performed gene array analysis. In SVZ after 4 days post lesin (dpl) LPC-injected tissue, demonstrated regulation of BMP pathway elements, including increased chordin, noggin, and ChorR, and decreased BMP4, compared to NaCl-injected tissue. GEArrayTM expression array systems (cat#OMM031 SuperArray, Bethesda, MD) consisted of spotted cDNA fragments encoding 113 mouse genes for neurotrophic signaling molecules involved in neuronal growth and differentiation, as well as regeneration and survival. Control sequences (PUC18, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), peptidylpropyl isomerase A (Ppia), and ?-actin) were also included. These microarrays were employed to compare SVZ gene expression 4 days after NaCl or LPC injection in corpus callosum. Total RNA was isolated by Trizol (Invitrogen) and processed for microarray hybridization following the manufacturer’s instructions. Arrays were visualized by autoradiography, and hybridization signals were scanned and analyzed for density in GEArray Expression Analysis Suite 2.0. The normalized value for each gene was calculated by dividing the value of each gene by the average value of the housekeeping genes GAPDH, Ppia, and ?-actin.

2010-04-13 | E-GEOD-21310 | biostudies-arrayexpress

Ganaxolone enhances microglial clearance activity and promotes remyelination in focal demyelination in the corpus callosum of ovariectomized rats.

Project description: Not available

| S-EPMC6978248 | biostudies-literature

Live imaging of remyelination in the adult mouse corpus callosum.

Project description: Not available

| S-EPMC8285919 | biostudies-literature

Proteomic changes during experimental de- and remyelination in the corpus callosum.

Project description: Not available

| S-EPMC7145428 | biostudies-literature

Single-cell RNA-Seq of mice treated with lysolecithin at baseline, 5 days post lesioning, 14 days post lesioning and 28 days post-lesioning, 3-4 months old

Project description:Here we studied the transcriptional changes in the oligodendrocyte lineage cells of corpus callosum in and around the region lesioned by injection of lysolecithin by using unbiased single cell RNA-seq. We identified three distinct activation states of oligodendrocytes, termed disease associated oligodendrocytes and associated gene expression signatures. We observed increases in proportion of disease associated oligodendrocytes along different timepoints post lysolecithin treatment. This study will provide resources to study the molecular mechanisms driving de/remyelination induced by lysolecithin.

2022-08-23 | GSE182846 | GEO

Adult-born SVZ progenitors receive transient synapses during remyelination in corpus callosum.

Project description: Not available

| S-EPMC4681435 | biostudies-literature