Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ATACseq of 3T3L1 cells undergoing differentiation

ABSTRACT: Confluency-arrested, undifferentiated 3T3-L1 cells were compared with DHA and Dexamethasone treated cells at 4 hrs to assess the effect of early differentiation on chromatin architecture.

ORGANISM(S): Homo sapiens

PROVIDER: GSE118470 | GEO | 2019/11/25

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

RNAseq of 3T3L1 cells undergoing differentiation

Project description:Confluency-arrested, undifferentiated 3T3-L1 cells were compared with differentiating cells at 24 hrs to assess the effect of differentiation on gene expression. Differentiation was induced by historical differentiation cocktail, ctrl (-DHA) differentiation cocktail, or DHA cocktail.

2019-11-25 | GSE118471 | GEO

PPARg dependent expression changes during differentiation of 3T3L1 cell into adipocytes

Project description:We profiled PPARg dependent gene expression changes during differntiation of 3T3L1 cell using PPARg siRNA 3T3-L1 (Pre-adipocyte) cell line was induced to differentiate using standard adipocyte differentiation media (IBMX, Dex and Insulin) 48hrs post-confluency. RNA was harvested at day -2 (confluent fibroblasts), 48hrs post-induction with IBMX, DEX and Insulin (day=0) and for each subsequent day after rosiglitazone treatment. Illumina beadchip microarrays were used to determine expression profiles of genes differentially regulated in cells transfected with either siRNA targeting PPARgamma or a non-targeting control siRNA.

2009-03-24 | GSE12929 | GEO

PPARg dependent expression changes during differentiation of 3T3L1 cell into adipocytes

2010-04-08 | E-GEOD-12929 | biostudies-arrayexpress

Promoter Capture Hi-C of day 0 (D0) and day 7 (D7) 3T3-L1 cells before and after adipogenic treatment

Project description:3T3-L1 preadipocytes were differentiated into mature adipocytes using adipogenic differentiation media. Promoter Capture Hi-C at D0 (two days post confluency) and D7 of adipocyte differentiation was performed to analyse regulatory interactions.

2023-06-17 | GSE234747 | GEO

High-throughput sequencing of 3T3-L1 cells with Kdm5c knockdown

Project description:One day prior to confluency, non-targeting (scramble control) siRNA or Kdm5c siRNA were transfected into 3T3-L1 cells and gene expression was assessed by RNA-seq at 24hr, 48hr, and 72hr post transfection.

2020-09-07 | GSE147997 | GEO

Chromatin accessiblity of 3T3-L1 cells with Kdm5c knockdown

Project description:One day prior to confluency, non-targeting (scramble control) siRNA or Kdm5c siRNA were transfected into 3T3-L1 cells and chromatin structure was assessed by ATAC-seq at 24hr, 48hr, and 72 hr post transfection.

2020-09-07 | GSE147994 | GEO

CAGE-seq of day 0 (D0) and day 7 (D7) 3T3-L1 cells before and after adipogenic treatment

Project description:3T3-L1 preadipocytes were differentiated into mature adipocytes using adipogenic differentiation media. Total RNA was isolated at D0 (two days post confluency) and D7 of adipocyte differentiation. nAnT-iCAGE sequencing was performed to analyse transcriptional start sites.

2023-06-17 | GSE234749 | GEO

Genome-wide DNA methylation mapping in preadipocytes

Project description:DNA methylation plays a crucial role in the regulation of gene transcription. In this study, using MeDIP-seq experiment, we report the mapping of DNA methylation in undifferentiated 3T3-L1 cells (preadipocytes). Examination of DNA methylation pattern in undifferentiated 3T3-L1 cells (preadipocytes)

2013-11-29 | E-GEOD-50992 | biostudies-arrayexpress

Effect of Ube3d knockout on gene expression in 3T3-L1 preadipocytes

Project description:To investigate the effect of UBE3D loss on 3T3-L1 differentiation, we generated Ube3d-KO 3T3-L1 cells. We then performed RNA sequencing on undifferentiated WT and KO cells to assess gene expression changes and alterations in RNA 3' end processing.

2022-11-22 | GSE202310 | GEO

Development of gene expression signatures for contact-inhibited and adipogenic-differentiated 3T3-L1 and NIH3T3 fibroblasts

Project description:Given previous works showing that, in the process of adipogenic differentiation of 3T3-L1 fibroblasts, the cells need to be cultured to confluency followed by additional incubation before initiating differentiation, we hypothesized that contact inhibition of proliferation (CIP) is requisite for making the cells prone to the differentiation. We screened upregulated genes in contact-inhibited 3T3-L1 fibroblasts, as well as NIH3T3 fibroblasts that are also sensitive to contact inhibition, by a whole genome microarray analysis. We also screened the genes that undergo rapid downregulation after the initiation of adipogenic differentiation.

2015-05-28 | GSE69320 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data