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Esrrb extinction triggers dismantling of naïve pluripotency and marks commitment to differentiation.

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE118907 | GEO | 2018/09/13

REPOSITORIES: GEO

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Esrrb extinction triggers dismantling of naive pluripotency and marks commitment to differentiation.

Project description: Not available

| S-EPMC6213284 | biostudies-literature

Esrrb extinction triggers dismantling of naïve pluripotency and marks commitment to differentiation [Microarray]

Project description:Self-renewal of embryonic stem cells (ESCs) cultured in serum-LIF is incomplete with some cells initiating differentiation. While this is reflected in heterogeneous expression of naive pluripotency transcription factors (TFs), the link between TF heterogeneity and differentiation is not fully understood. Here we purify ESCs with distinct TF expression levels from serum-LIF cultures to uncover early events during commitment from naïve pluripotency. ESCs carrying fluorescent Nanog and Esrrb reporters show Esrrb downregulation only in NANOGlow cells. Independent Esrrb reporter lines demonstrate that ESRRBnegative ESCs cannot effectively self-renew. Upon ESRRB loss, pre-implantation pluripotency gene expression collapses. ChIP-Seq identifies different regulatory element classes that bind both OCT4 and NANOG in ESRRBhigh cells. Class I elements lose NANOG and OCT4 binding in ESRRBnegative ESCs and associate with genes expressed preferentially in naïve ESCs. In contrast, class II elements retain OCT4 but not NANOG binding in ESRRBnegative cells and associate with more broadly expressed genes. Therefore, mechanistic differences in TF function act cumulatively to restrict potency during exit from naïve pluripotency.

2018-09-13 | GSE118906 | GEO

Esrrb extinction triggers dismantling of naïve pluripotency and marks commitment to differentiation [ChIP-seq]

2018-09-13 | GSE118905 | GEO

Esrrb extinction triggers dismantling of naïve pluripotency and marks commitment to differentiation.

Project description:Esrrb extinction triggers dismantling of naïve pluripotency and marks commitment to differentiation.

| PRJNA487255 | ENA

Esrrb extinction triggers dismantling of naïve pluripotency and marks commitment to differentiation [Microarray]

Project description:Esrrb extinction triggers dismantling of naïve pluripotency and marks commitment to differentiation [Microarray]

| PRJNA487260 | ENA

Esrrb extinction triggers dismantling of naïve pluripotency and marks commitment to differentiation [ChIP-seq]

Project description:Esrrb extinction triggers dismantling of naïve pluripotency and marks commitment to differentiation [ChIP-seq]

| PRJNA487261 | ENA

Pluripotency-associated miR-290/302 family of microRNAs promote the dismantling of naive pluripotency.

Project description: Not available

| S-EPMC4783473 | biostudies-literature

The developmental dismantling of pluripotency is reversed by ectopic Oct4 expression.

Project description: Not available

| S-EPMC3367440 | biostudies-literature

m6A mRNA Methylation Facilitates Resolution of Naïve Pluripotency Towards Differentiation

Project description:This SuperSeries is composed of the SubSeries listed below.

2014-11-02 | GSE61998 | GEO

The combined action of Esrrb and Nr5a2 is essential for naïve pluripotency.

Project description:The maintenance of pluripotency in mouse embryonic stem cells (ESCs) is governed by the action of an interconnected network of transcription factors. Among them, only Oct4 and Sox2 have been shown to be strictly required for the self-renewal of ESCs and pluripotency, particularly in culture conditions where differentiation cues are chemically inhibited. Here, we report that the conjunct activity of two orphan nuclear receptors, Esrrb and Nr5a2, parallels the importance of that of Oct4 and Sox2 in naïve ESCs. By occupying a large common set of regulatory elements, these two factors control the binding of Oct4, Sox2 and Nanog to DNA. Consequently, in their absence the pluripotency network collapses and the transcriptome is substantially deregulated, leading to the differentiation of ESCs. Altogether, this work identifies orphan nuclear receptors, previously thought to be performing supportive functions, as a new set of core regulators of naïve pluripotency.

2021-05-04 | GSE152186 | GEO

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