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Maps of histone variant H3.V deposition in Trypanosoma brucei brucei

ABSTRACT: Chromatin immunoprecipitation of genomic loci in Trypanosoma brucei where histone variant H3.V is deposited. This was achieved by deletion of one H3.V allele and N-terminal tagging of the second H3.V allele with a Ty1 tag. During the ChIP experiment, the DNA was digested with MNase to obtain mononucleosomes. Nucleosomes containing H3.V were pulled down by using a BB2 anti-Ty1 antibody. Cross links are reversed and mononucleosomal DNA is purified and prepared for Illumina sequencing.

ORGANISM(S): Trypanosoma brucei brucei

PROVIDER: GSE118917 | GEO | 2018/08/24

REPOSITORIES: GEO

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