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Transcriptomics Expression Analysis of the effects of UV radiations on Normal Human Dermal Fibroblast Cells

ABSTRACT: Chronic exposure to sunlight is linked to premature aging (photoaging) and UV radiations is implicated to be the main contributor of this phenomenon. It causes changes to dermal collgen through two major pathways; promotion of collagen breakdown and lost of collagen content via the inhibition of collagen biosynthesis. The absorption of UV radiations by skin molecules generates reactive oxygen species (ROS) which lead to oxidative damage of cellular components such as cell walls, lipid membranes, mitochondria and DNA. Therefore, this research involve the identification of key genes in biological processes through high throughput RNA Sequencing technology to identify genes and pathways underlying skin aging health and diseases due to UV exposure, ultimately leading to novel ways of managing, diagnose, treat and prevent/slow down skin aging illnesses/wrinkles.The results from this study produces huge amount of differential expressed genes (DEG) as a result of UV exposure which were subsquently validated with qPCR.

ORGANISM(S): Homo sapiens

PROVIDER: GSE119009 | GEO | 2021/07/01

REPOSITORIES: GEO

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Single-cell RNA-seq of UVB-radiated Skin Reveals Landscape of Photoaging-related Inflammation and Protection by Vitamin D

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Effect of rice fermentation and succinic acid on gene expression of human dermal fibroblasts irradiated with UVA

Project description:Skin aging caused by UV is called photoaging, which is characterized by deeper wrinkles accompanied by senescence of dermal fibroblasts and reduction of collagens. Rice fermentation, widely used in the cosmetics, has been reported to possess anti-aging benefits on skincare, however, its roles in the skin photoaging remains unclear. In this study, the effects of Maifuyin (a rice fermentation), and its ingredients succinic acid (SA) and choline on UVA-induced senescence in fibroblasts were evaluated. A mRNA sequencing technology (RNA-seq) was applied to study the effects of these ingredients on UVA-induced photoaging. In conclusion, these results highlight the potential use of Maifuyin and SA as promising agents for anti-photoaging applications.

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Solar simulated ultraviolet radiation induces histone 3 methylation changes in the gene promoters of matrix metalloproteinases 1 and 3 in primary human dermal fibroblasts [II]

2014-08-27 | E-GEOD-60795 | biostudies-arrayexpress

Solar simulated ultraviolet radiation induces histone 3 methylation changes in the gene promoters of matrix metalloproteinases 1 and 3 in primary human dermal fibroblasts [I]

2014-08-27 | E-GEOD-60794 | biostudies-arrayexpress

Changes of genes in time-dependent photoaged mouse model

Project description:Photoaging results from the damaging effects of long-term exposure to UV. It is characterized by deep wrinkle, but the mechanism is still lack. To better understand molecular events contributing to photoaging, We used microarray analysis using an optimized in time-dependent wrinkle model in mice.

2014-07-14 | GSE58915 | GEO

Gene-expression profiling of Saccharomyces cerevisiae irradiated by high-LET radiations

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