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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and FXRalpha-/- testicular Transcriptomes with Environmental Exposure

ABSTRACT: The goals of this study are to define the putatitve impacts of FXRalpha deficiency in testicular physiology following environmental exposure (bisphenol-A and stigmasterol)

ORGANISM(S): Mus musculus

PROVIDER: GSE119012 | GEO | 2018/12/01

REPOSITORIES: GEO

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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and FXRalpha-/- testicular Transcriptomes

Project description:The goals of this study are to define the putatitve impacts of FXRalpha deficiency in testicular physiology

2017-07-28 | GSE84945 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and L3mbtl2 cKO Testicular Transcriptomes

Project description:Our study represents the first detailed analysis of testicular transcriptomes, with biologic replicates, generated by RNA-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of expression profiles.

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Genes responsive to bisphenol A in testicular Sertoli cells

Project description:We performed global scale microarray analysis to identify detailed mechanisms by which bisphenol A (BPA) induce cell death by using an Affymetrix GeneChip system. Testicular Sertoli TTE3 cells used in the present study were derived from transgenic mice harboring a temperature-sensitive simian virus 40 large T-antigen. Cell death accompanying endoplasmic reticulum stress was observed in the cells treated with 0.2 mM BPA. Of the 22,690 probe sets analyzed, approximately 1,300 genes were down- and up-regulated by a factor of 2.0 or greater in the cells treated with BPA. Keywords: bisphenol A, gene expression, testicular Sertoli cell

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Next Generation Sequencing Facilitates Quantitative Analysis of Normal Human Kidney Transcriptomes

Project description:Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived normal human kidney transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis The kidney tissue was immediately placed and stored in RNAlater® (Ambion), according to the manufacturer’s instruction. The tissue was manually microdissected under microscope in RNAlater® pool for glomerular and tubular compartment. Dissected tissue was homogenized and RNA was prepared using RNAeasy mini columns (Qiagen, Valencia, CA, US), according to the manufacturer’s instructions. RNA quality and quantity were determined using the Laboratory-on-Chip Total RNA PicoKit Agilent BioAnalyzer. Only samples without evidence of degradation were further used (RNA Integrity Number >6).

2014-08-06 | E-GEOD-60119 | biostudies-arrayexpress

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and FXRalpha-/- testicular Transcriptomes with Environmental Exposure

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and FXRalpha-/- testicular Transcriptomes with Environmental Exposure

| PRJNA487776 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of testis Transcriptomes in Xenopus laevis

Project description:Purpose: Circular RNAs (circRNAs) are implicated in multiple developmental anomalies. The goals of this study are to research whether circRNAs involve in the effects in atrazine (AZ)-exposed X. laevis Methods: At Nieuwkoop-Faber (NF) stage 47 (13 d post-hatch), mixed sex X. laevis tadpoles exposed to 100 µg/L AZ for 180 days. Then total RNAs from individual testis was isolated. RNA library construction and circRNA sequencing was analysis Results: CircRNAs were detected and identified with Find_circ software. We found that treatment of AZ led to 405 circRNAs differentially expressed including 44 upregulated and 361 downregulated compared with froglets in the control groups. Two hundreds and eighty two differentially expressed circRNAs played miRNA sponges roles. Kyoto Encyclopedia of Genes and Genomes pathway analysis of miRNA targets showed that AZ-affected circRNAs are mainly involved in 19 pathways Conclusions: This study for the fisrt time provides evidence that AZ can alter circRNAs which play a role in AZ-induced testicular degeneration of developing male X. laevis through regulation of expressions of functional genes in the testes of X. laevis.

2019-09-01 | GSE103526 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of ISL1 Regulated Transcriptomes

Project description:The goals of this study are to analyze the transcriptome profiling (RNA-seq) after ISL1 overexpression in Huh7 cells.

2019-02-01 | GSE77958 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of PBX3 Regulated Transcriptomes

Project description:The goals of this study are to analyze the transcriptome profiling (RNA-seq) after PBX3 overexpression in SMMC-7721 cells.

2015-09-24 | GSE71261 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of IBP Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived strain GCY transcriptome profiling (RNA-seq) and to evaluate protocols for optimal high-throughput data analysis

2021-09-08 | GSE183578 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of CHAA Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived strain JSTW transcriptome profiling (RNA-seq) and to evaluate protocols for optimal high-throughput data analysis

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