Genomics

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Transcriptome analysis of influenza infected GFP+ AEC compared to bystander GFP- AEC


ABSTRACT: A GFP-expressing recombinant A/Puerto Rico/8/1934 influenza virus was used to infect C57BL/6 wild type mice and on day 3 post infection, lung alveolar epithelial cells (AEC) were isolated and sorted based on GFP expression. GFP+ AEC represent the infected AEC and GFP- AEC represent the bystander AEC. AEC were also sorted from uninfected mice to serve as controls. Overall design: AEC from infected mice were pooled to make three (3) infected GFP+ AEC replicates for sequencing. Five (5) bystander GFP- replicates and five (5) uninfected AEC replicates were also isolated for sequencing

INSTRUMENT(S): Illumina HiSeq 2000 (Mus musculus)

SUBMITTER: Peter Katsikis  

PROVIDER: GSE119123 | GEO | 2018-08-29

REPOSITORIES: GEO

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