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SFP1 (ChIP-seq)

ABSTRACT: We set out to determine how Sfp1 binding targets evolved over time. We sampled our species of interest to include one pre-whole genome duplicaton (WGD) species containing SFP1A (K. lactis), one post-WGD species containing both SFP1 and SFP1PL (N. castellii), two post-WGD species containing only SFP1 (S. cerevisiae and S. paradoxus) and performed ChIP for each Sfp1 homolog in each species using an antibody that recognizes a conserved subdomain of Sfp1 common to both Sfp1, Sfp1a, and Sfp1pl. Because Sfp1pl was also present in N. castellii, a second antibody was used that is more specific to Sfp1pl (denoted as IP3). We also performed ChIP in SFP1 deletion mutants in each species tested, as a control."

ORGANISM(S): Naumovozyma castellii Saccharomyces cerevisiae Kluyveromyces lactis Saccharomyces paradoxus

PROVIDER: GSE119211 | GEO | 2020/08/29

REPOSITORIES: GEO

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A comparative gene expression data set for functional Sfp1 knockdown mutants and wild type strains for five yeast Species in the LOG growth phase

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SFP1 (ChIP-seq)

Project description:SFP1 (ChIP-seq)

| PRJNA488473 | ENA

SFP1 (RNA-seq)

Project description:SFP1 (RNA-seq)

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Transcription profiling of S. cerevisiae S. cerevisiae strain CEN.PK113.7D and the isogenic sfp1 null mutant were grown in chemostat cultures under ethanol(aerobic)- and glucose(anaerobic)- limitation to determine SFP1 dependent transcription

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